NPR2 / YEL062W Overview
- Standard Name
- NPR2 1
- Systematic Name
- YEL062W
- SGD ID
- SGD:S000000788
- Feature Type
- ORF , Verified
- Description
- Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2 1 2 3 4 5 6 7 8 9
- Name Description
- Nitrogen Permease Regulator 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 615
- Mol. Weight (Da)
- 69868.4
- Isoelectric Point
- 8.69
- Median Abundance (molecules/cell)
- 1643 +/- 727
- Half-life (hr)
- 7.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NPR2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of SEACIT, a subcomplex of the SEA complex that acts as a GTPase-activating protein (GAP) to negatively regulates signaling through the heterodimeric Gtr1p-Gtr2p GTPase; negative regulator of TORC1 signaling in response to amino acid deprivation; positive regulator of autophagy and autophagosome formation; loosely associated with the vacuolar membrane
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in cellular response to amino acid starvation (IMP)
- involved in cellular response to nitrogen starvation (IGI, IMP)
- involved in negative regulation of TORC1 signaling (IGI, IMP)
- involved in positive regulation of autophagy (IGI, IMP)
- involved in proline transport (IMP)
- involved in regulation of autophagosome assembly (IMP)
- involved in response to xenobiotic stimulus (IMP)
- involved in urea transport (IMP)
Cellular Component
- Manually Curated
- part of GATOR1 complex (IDA)
- part of Seh1-associated complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows abnormal localization of Tor1p and increased phosphorylation of Sch9p; null mutation results in defective autophagy and lower utilization of proline, ammonium or urea as nitrogen source; null mutant is resistant to cisplatin and doxorubicin; homozygous null mutant diploid has defects in meiosis and sporulation; in systematic studies null mutant is sensitive to azole drugs, tunicamycin, DTT, camptothecin, but resistant to bleomycin and caffeine
Classical Genetics
- null
- autophagy: decreased
- cell death: increased rate
- chemical compound accumulation: increased
- meiosis: decreased
- metal resistance: decreased
- mutation frequency: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: decreased
- utilization of nitrogen source: decreased
- unspecified
- null
- acid pH resistance: decreased
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound excretion: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- innate thermotolerance: decreased
- metal resistance: decreased
- metal resistance: increased
- protein/peptide accumulation: decreased
- protein/peptide distribution: abnormal
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- sporulation efficiency: decreased
- sporulation: absent
- stress resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
206 total interactions for 165 unique genes
Physical Interactions
- Affinity Capture-MS: 35
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 16
- Co-localization: 1
- Two-hybrid: 2
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 100
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 14
- Positive Genetic: 16
- Synthetic Growth Defect: 4
- Synthetic Lethality: 8
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.