UBP3 / YER151C Overview
- Standard Name
- UBP3 1
- Systematic Name
- YER151C
- SGD ID
- SGD:S000000953
- Aliases
- BLM3 17
- Feature Type
- ORF , Verified
- Description
- Ubiquitin-specific protease that cleaves Ub-protein fusions; interacts with cofactor Bre5p to regulate transport between ER and Golgi, deubiquitinating COPII and COPI proteins, Sec23p and Sec27p; mediates steady-state retention of Golgi membrane proteins with Bre5p; phosphorylation (S695) by Hog1p regulates transcriptional activation by osmostress; inhibits Ras/PKA signaling through Ira2p; inhibits silencing; role in ribophagy; protein abundance increases in response to DNA replication stress 1 2 3 4 5 6 7 8 9 10
- Name Description
- UBiquitin-specific Protease 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 912
- Mol. Weight (Da)
- 101918.9
- Isoelectric Point
- 7.97
- Median Abundance (molecules/cell)
- 6584 +/- 2579
- Half-life (hr)
- 10.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all UBP3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- A thiol-dependent ubiquitin-specific protease that is a component of the Ubp3-Bre5 deubiquitination complex; binds mRNA and regulates the osmotic stress response and ER to Golgi vesicle-mediated transport; also involved in ribophagy; localized to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables cysteine-type deubiquitinase activity (IMP, IDA)
- enables mRNA binding (HDA, IDA)
Biological Process
- Manually Curated
- involved in protein deubiquitination (IMP, IDA)
- involved in protein retention in Golgi apparatus (IMP, IGI)
- involved in regulation of ER to Golgi vesicle-mediated transport (IMP)
- involved in regulation of response to osmotic stress (IMP, IPI)
- involved in ribophagy (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, have large cell size, are defective in vacuolar fragmentation, impaired in use of various nitrogen sources, resistant to DNA-damaging agent methyl methanesulfonate, and senstive to heat, anoxia, desiccation, caffeine, metals, butanol, various antibiotics including TOR inhibitor rapamycin, DNA topoisomerase inhibitor camptothecin, DNA synthesis inhibitor hydroxyurea, and protein synthesis inbibitor cycloheximide; ; overexpression enhances pseudohyphal growth in Sigma1278b, slows growth in S288C
Classical Genetics
- null
- chemical compound accumulation: increased
- heat sensitivity: increased
- metal resistance: decreased
- oxidative stress resistance: decreased
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- ribophagy: decreased
- viability: decreased
- overexpression
- unspecified
- reduction of function
- null
- anaerobic growth: decreased
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chromosome/plasmid maintenance: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- haploinsufficient
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- killer toxin resistance: decreased
- metal resistance: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- toxin resistance: decreased
- transposable element transposition: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
2026 total interactions for 1201 unique genes
Physical Interactions
- Affinity Capture-MS: 537
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 32
- Biochemical Activity: 13
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 7
- PCA: 1
- Proximity Label-MS: 3
- Reconstituted Complex: 5
- Two-hybrid: 9
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 13
- Negative Genetic: 1088
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 20
- Positive Genetic: 154
- Synthetic Growth Defect: 93
- Synthetic Lethality: 14
- Synthetic Rescue: 12
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-02-15
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.