FZF1 / YGL254W Overview
- Standard Name
- FZF1 1
- Systematic Name
- YGL254W
- SGD ID
- SGD:S000003223
- Aliases
- NRC299 , RSU1 7 , SUL1 6
- Feature Type
- ORF , Verified
- Description
- Transcription factor involved in sulfite metabolism; activates transcription of SSU1, DDI2, DDI3; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress 1 2 3 4 5
- Name Description
- Five Zinc Fingers 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 299
- Mol. Weight (Da)
- 33996.5
- Isoelectric Point
- 7.9
- Median Abundance (molecules/cell)
- 2120 +/- 955
- Half-life (hr)
- 6.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all FZF1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence specific DNA binding protein involved in positive regulation of sulfite transport by positive regulation of transcription from RNA polymerase II promoter; localizes to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription activator activity, RNA polymerase II-specific (IMP, IDA)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA, IMP)
- enables sequence-specific DNA binding (HDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cyanamide metabolic process (IMP)
- involved in positive regulation of sulfite transmembrane transport (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- unspecified
- reduction of function
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
115 total interactions for 107 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 4
- Biochemical Activity: 2
- Co-localization: 1
- Protein-RNA: 1
- Two-hybrid: 8
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 3
- Negative Genetic: 81
- Phenotypic Suppression: 1
- Positive Genetic: 5
- Synthetic Growth Defect: 3
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- FZF1 encodes a DNA-binding transcription factor involved in the regulation of sulfite metabolism. Fzf1p contains five zinc fingers of the Cys2His2 class, three of which are positioned in tandem at the N-terminus. The fourth and fifth zinc fingers follow after interruptions of 61 and 66 amino acids, respectively. The Fzf1p binding site 5'-CTATCA-3' is present at thousands of sites throughout the genome, but the sole Fzf1p target identified thus far is SSU1, which encodes a plasma membrane sulfite pump. Fzf1p binds directly to the SSU1 promoter, and its first zinc finger region is absolutely required for DNA binding. Disruptions of Fzf1p structure can confer sulfite resistance through hyperactivation of SSU1.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-02-25
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.