PIL1 / YGR086C Overview
- Standard Name
- PIL1 1 2
- Systematic Name
- YGR086C
- SGD ID
- SGD:S000003318
- Feature Type
- ORF , Verified
- Description
- Eisosome core component involved in endocytosis; mitochondria phosphoprotein that localizes to the outer membrane and is involved in maintaining mitochondrial morphology and mitophagy; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress 1 3 4 5 6 7 8
- Name Description
- Phosphorylation Inhibited by Long chain bases 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mitochondrial phosphoprotein; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress
- Length (a.a.)
- 339
- Mol. Weight (Da)
- 38319.3
- Isoelectric Point
- 4.26
- Median Abundance (molecules/cell)
- 29832 +/- 9050
- Half-life (hr)
- 6.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PIL1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Lipid-binding protein involved in protein localization, endocytosis and eisosome assembly; also involved in negative regulation of protein kinase activity and the response to heat; localizes to the eisosome
View computational annotations
Molecular Function
- Manually Curated
- enables lipid binding (IDA)
Biological Process
- Manually Curated
- involved in eisosome assembly (IMP)
- involved in endocytosis (IMP)
- involved in protein localization (IMP)
- involved in protein localization to eisosome filament (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in cytoplasm (HDA)
- located in eisosome (IDA)
- located in mitochondrial outer membrane (IDA, HDA)
- located in mitochondrion (HDA)
- located in plasma membrane (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has an abnormal plasma membrane, with defective eisosome organization and aberrant localization of eisosome proteins; null mutant is sensitive to rapamycin and H2O2, has reduced endocytosis, and has increased innate thermotolerance; in large-scale studies, null mutant has reduced competitive fitness in a variety of different media, is sensitive to osmotic stress, and has altered resistance to a variety of chemicals
Classical Genetics
- null
- autophagy: decreased
- chemical compound accumulation: increased
- endocytosis: decreased rate
- hyperosmotic stress resistance: decreased
- innate thermotolerance: increased
- mitochondrial morphology: abnormal
- mitophagy: decreased
- necrotic cell death: increased
- oxidative stress resistance: decreased
- plasma membrane morphology: abnormal
- protein secretion: increased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide distribution: decreased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- overexpression
- reduction of function
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- hyperosmotic stress resistance: decreased
- protein activity: increased
- protein/peptide distribution: abnormal
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- viable
- overexpression
- reduction of function
- acid pH resistance: increased
- heat sensitivity: increased
- metal resistance: decreased
- metal resistance: increased
- osmotic stress resistance: decreased
- osmotic stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- UV resistance: increased
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
822 total interactions for 618 unique genes
Physical Interactions
- Affinity Capture-MS: 535
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 9
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-localization: 2
- Co-purification: 1
- Cross-Linking-MS (XL-MS): 1
- FRET: 3
- PCA: 7
- Proximity Label-MS: 5
- Reconstituted Complex: 1
- Two-hybrid: 9
Genetic Interactions
- Dosage Growth Defect: 3
- Negative Genetic: 176
- Phenotypic Enhancement: 11
- Phenotypic Suppression: 4
- Positive Genetic: 31
- Synthetic Growth Defect: 6
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 1
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 14
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.