SKN7 / YHR206W Overview
- Standard Name
- SKN7 1
- Systematic Name
- YHR206W
- SGD ID
- SGD:S000001249
- Aliases
- BRY1 9 , POS9 10
- Feature Type
- ORF , Verified
- Description
- Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication 2 3 4 6 7 8
- Name Description
- Suppressor of Kre Null 5
- Paralog
- HMS2 7
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- SKN7/YHR206W is located toward the end of the right arm of chromosome VIII between SCH9 protein kinase and SET5 histone methyltransferase; coding sequence is 1869 nucleotides long with 5 SNPs, one of which causes an Ala/Val polymorphism at residue 584; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Skn7p is 622 amino acids long, short-lived, low in abundance; contains winged helix DNA-binding domain; phosphorylated on 9 residues; relocalizes to the cytosol in response to hypoxia
- Length (a.a.)
- 622
- Mol. Weight (Da)
- 69199.1
- Isoelectric Point
- 7.05
- Median Abundance (molecules/cell)
- 3141 +/- 571
- Half-life (hr)
- 5.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SKN7 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor that regulates transcription from RNA polymerase II promoter in response to oxidative stress; also involved in regulation of cell size, and responses to osmotic stress and singlet oxygen; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor activity (IDA, IMP)
- enables phosphorelay response regulator activity (IDA, IMP)
- enables sequence-specific DNA binding (HDA)
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IMP)
- involved in regulation of cell size (IMP)
- involved in regulation of endoplasmic reticulum unfolded protein response (IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in response to singlet oxygen (IMP)
- involved in transcription elongation by RNA polymerase II (IDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants have decreased ethanol tolerance, are sensitive to oxidative stress and dessication, show delayed S phase progression, increased mutation frequency, decreased filamentous growth under nitrogen starvation, and increased chitin deposition; overexpression slows growth, interferes with budding and cellular morphology
Classical Genetics
- null
- cell cycle progression in S phase: decreased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chitin deposition: increased
- chromosome/plasmid maintenance: abnormal
- filamentous growth: decreased
- mitotic recombination: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- survival rate in stationary phase: decreased
- overexpression
- null
- auxotrophy
- cell cycle progression in S phase: delayed
- chemical compound accumulation: abnormal
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- mutation frequency: increased
- oxidative stress resistance: decreased
- redox state: abnormal
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- stress resistance: decreased
- toxin resistance: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Skn7p interacts physically with proteins involved in transcription; SKN7 interacts genetically with genes involved in transcription; the skn7 null mutant is viable, the null mutant of paralog hms2 is viable, the skn7 hms2 double mutant has not been annotated for phenotype
455 total interactions for 283 unique genes
Physical Interactions
- Affinity Capture-MS: 14
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 8
- Biochemical Activity: 5
- Co-purification: 1
- PCA: 2
- Protein-peptide: 1
- Reconstituted Complex: 4
- Two-hybrid: 31
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Dosage Rescue: 11
- Negative Genetic: 288
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 5
- Positive Genetic: 31
- Synthetic Growth Defect: 29
- Synthetic Lethality: 4
- Synthetic Rescue: 8
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- SKN7 encodes a highly-conserved kinase-regulated stress-responsive transcription factor that modulates the response to oxidative insults and cell wall stress. Skn7p consists of an N-terminal DNA-binding domain similar to that of the heat shock transcription factor (Hsf1p) and a C-terminal receiver domain which confers regulation of Skn7p transcriptional activity by His-Asp phosphorelay signaling via phosphorylation of a conserved aspartate. Both the DNA-binding domain and the receiver domain are essential for the role of Skn7p in the regulation of cell wall biosynthesis, the cell cycle, and the response to osmotic shock and to oxidative stress. Skn7p activates transcription of many of the key oxidative stress response genes, including TRR1, TRX2, TSA1, GPX2, AHP1, CCP1, and CTT1. Skn7p also upregulates heat shock proteins by binding to heat shock elements (HSEs) in H2O2-treated cells exposed to heat shock.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.