HAP4 / YKL109W Overview
- Standard Name
- HAP4 1
- Systematic Name
- YKL109W
- SGD ID
- SGD:S000001592
- Feature Type
- ORF , Verified
- Description
- Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift 1 2
- Name Description
- Heme Activator Protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Hap4p is 554 amino acids long, low in abundance, has shorter half-life in petite cells; sumoylated on K150, phosphorylated on 3 residues
- Length (a.a.)
- 554
- Mol. Weight (Da)
- 62389.6
- Isoelectric Point
- 5.12
- Median Abundance (molecules/cell)
- 790 +/- 368
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HAP4 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Transcriptional coactivator; binds RNA polymerase II transcription factors to activate gene expression in response to carbon catabolites; subunit of the CCAAT-binding transcription factor complex
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of cellular respiration (IMP)
Cellular Component
- Manually Curated
- part of CCAAT-binding factor complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants grow slowly, have decreased ethanol tolerance, increased lifespan under caloric restriction, respiration defects, decreased filamentous growth, cannot utilize some nitrogen sources, small cell size and abnormally elongated buds; null is also sensitive to UV, dessication, and anoxia; homozygous diploid null has sporulation defects; overexpression slows vegetative growth
Classical Genetics
- null
- overexpression
- reduction of function
- null
- anaerobic growth: decreased
- bud morphology: abnormal
- cell size: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- filamentous growth: decreased
- haploinsufficient
- invasive growth: absent
- mitophagy: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- utilization of carbon source: decreased
- utilization of nitrogen source: decreased rate
- UV resistance: decreased
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
348 total interactions for 296 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 8
- Reconstituted Complex: 8
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Dosage Rescue: 7
- Negative Genetic: 252
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 15
- Positive Genetic: 32
- Synthetic Growth Defect: 1
- Synthetic Lethality: 1
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- HAP4 encodes an alpha helix transcription factor that provides the principal activation function of the heteromeric HAP2/3/4/5 complex which binds CCAAT boxes upstream of genes involved in respiration and oxidative phosphorylation. The Hap2/3/4/5 complex plays a central role in converting cells from fermentative to respiratory growth following the diauxic shift by inducing genes required for mitochondrial function upon glucose depletion. The HAP2/3/4/5 complex stimulates mitochondrial biogenesis in high oxygen and represses hypoxic genes. Hap2p, Hap3p, and Hap5p are constitutively expressed and together form a DNA-binding complex. Hap4p is induced upon glucose depletion, and provides the activation domain of the complex. Increased expression of Hap4 alone yields induction of genes under regulatory control of the HAP2/3/4/5 complex.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.