TIS11 / YLR136C Overview
- Standard Name
- TIS11 1
- Systematic Name
- YLR136C
- SGD ID
- SGD:S000004126
- Aliases
- CTH2 2
- Feature Type
- ORF , Verified
- Description
- mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; represses mitochondrial function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication 1 2 3 4 5 6 7
- Name Description
- similar to the mammalian TPA Induced Sequence gene family 1
- Paralog
- CTH1 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Tis11p is 285 amino acids long and low in abundance; relative distribution to the nucleus increases upon DNA replication stress; has 2 zinc-finger domains; phosphorylated on 5 serines
- Length (a.a.)
- 285
- Mol. Weight (Da)
- 32325.9
- Isoelectric Point
- 9.26
- Median Abundance (molecules/cell)
- 2074
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all TIS11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- mRNA-binding translation repressor involved in mRNA catabolism and iron homeostasis; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (IDA)
- enables translation repressor activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in electron transport coupled proton transport (IDA)
- involved in intracellular iron ion homeostasis (IMP)
- involved in negative regulation of cytoplasmic translation (IDA)
- involved in nuclear-transcribed mRNA catabolic process (IMP)
- involved in response to iron ion starvation (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null grows slowly, shows small defect in vacuolar fragmentation, defect in endocytosis, increased resistance to MMS, increased replicative and chronological lifespans, decreased desiccation resistance, increased competitive fitness; overexpression slows growth, reduces resistance to hydroxyurea and fluconazole, increases invasive growth
Classical Genetics
- null
- chronological lifespan: increased
- competitive fitness: increased
- desiccation resistance: decreased
- endocytosis: decreased
- heat sensitivity: decreased
- metal resistance: decreased
- oxidative stress resistance: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Tis11p interacts physically with proteins involved in RNA catabolism; TIS11 interacts genetically with genes involved in transcription; the tis11 null mutant is viable; the null mutant of paralog cth1 is viable; the tis11 cth1 double mutant displays a phenotypic enhancement
81 total interactions for 67 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 2
- Biochemical Activity: 1
- Co-purification: 1
- PCA: 3
- Two-hybrid: 10
Genetic Interactions
- Negative Genetic: 42
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 4
- Positive Genetic: 6
- Synthetic Growth Defect: 1
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- TIS11 transcription is downregulated by Rim101p during mitosis; TIS11 promoter is bound by Met32p and Met4p; TIS11 promoter is bound by Bur6p, Gcn5p, Med6p, and Xbp1p in response to heat; TIS11 transcription is regulated by Opi1p, Pdr1p, Srb5p, and Stp1p; TIS11 transcription is regulated by Sfp1p in response to stress; TIS11 transcription is upregulated by Zap1p in response to zinc starvation
- Regulators
- 14
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.