CDC73 / YLR418C Overview
- Standard Name
- CDC73 1
- Systematic Name
- YLR418C
- SGD ID
- SGD:S000004410
- Feature Type
- ORF , Verified
- Description
- Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; role in preventing L-A mycovirus pathogenesis; protein abundance increases in response to DNA replication stress; human homolog, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers 2 3 4 5 6 7 8 9 10 11
- Name Description
- Cell Division Cycle 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 393
- Mol. Weight (Da)
- 44457.7
- Isoelectric Point
- 8.1
- Median Abundance (molecules/cell)
- 5367 +/- 1452
- Half-life (hr)
- 10.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC73 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Non-DNA binding transcription factor; subunit of the Cdc73/Paf1 transcription elongation complex; involved in transcription activation from Pol I and II promoters, regulation of transcription coupled nucleotide excision repair (NER), mRNA 3'-end processing and histone H3 methylation
View computational annotations
Molecular Function
- Manually Curated
- enables chromatin binding (IDA)
- enables RNA polymerase II C-terminal domain phosphoserine binding (IDA)
- enables RNA polymerase II complex binding (IPI)
Biological Process
- Manually Curated
- involved in mRNA 3'-end processing (IMP)
- involved in negative regulation of DNA recombination (IMP)
- involved in positive regulation of transcription elongation by RNA polymerase I (IDA)
- involved in positive regulation of transcription elongation by RNA polymerase II (IMP)
- involved in regulation of transcription-coupled nucleotide-excision repair (IGI)
- involved in transcription elongation by RNA polymerase I (IMP)
- involved in transcription elongation by RNA polymerase II (IGI)
Cellular Component
- Manually Curated
- part of Cdc73/Paf1 complex (IPI)
- located in euchromatin (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- cold sensitivity: increased
- colony sectoring: increased
- heat sensitivity: increased
- oxidative stress resistance: decreased
- petite-negative
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- null
- auxotrophy
- bud morphology: abnormal
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- colony appearance: abnormal
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- liquid culture appearance: abnormal
- metal resistance: decreased
- mitochondrial genome maintenance: abnormal
- mitochondrial morphology: abnormal
- mutation frequency: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: decreased
- telomere length: decreased
- transposable element transposition: increased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1838 total interactions for 1028 unique genes
Physical Interactions
- Affinity Capture-MS: 141
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 39
- Co-fractionation: 1
- Co-purification: 4
- Cross-Linking-MS (XL-MS): 1
- PCA: 7
- Protein-RNA: 4
- Reconstituted Complex: 16
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 1
- Negative Genetic: 780
- Phenotypic Enhancement: 21
- Phenotypic Suppression: 6
- Positive Genetic: 204
- Synthetic Growth Defect: 206
- Synthetic Lethality: 396
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- CDC73 encodes a component of the Polymerase-Associated Factor 1 (Paf1) complex (Paf1C). Other members of Paf1C include Ctr9p, Leo1p, Paf1p, and Rtf1p. Paf1C is highly conserved throughout eukaryotes and participates in multiple aspects of RNA polymerase II (RNAPII) transcriptional regulation. Paf1c promotes RNAPII transcription elongation and transcription-coupled histone modifications, thereby affecting various cellular processes such as gene expression, silencing, RNA maturation, DNA repair, and cell cycle progression. CDC73 was originally identified in a genetic screen for cell cycle regulators, and Cdc73p plays a key role in recruiting Paf1C to chromatin. The C-terminal Ras-like domain of Cdc73p is the most highly conserved part of the protein, and is crucial for full recruitment of Paf1C to active genes. The C-terminal domain of Cdc73p binds the RNAPII C-terminal domain (CTD). Cdc73p is also needed for trimethylation of histone H3K36 by the Set2p methyltransferase, affecting histone acetylation levels. Paf1C influences gene expression by promoting histone H2BK123 ubiquitylation and methylation at histone H3K4 and K79. Paf1C functions cooperatively with other factors influencing chromatin structure, such as the elongation complex Spt4p-Spt5p, the histone chaperone FACT, and the chromatin remodeler Chd1p. Paf1C also coordinates transcription elongation with transcription termination and RNA 3'-end processing.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.