PHO84 / YML123C Overview
- Standard Name
- PHO84
- Systematic Name
- YML123C
- SGD ID
- SGD:S000004592
- Aliases
- phoT 6
- Feature Type
- ORF , Verified
- Description
- High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity 1 2 3 4 5
- Name Description
- PHOsphate metabolism
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 587
- Mol. Weight (Da)
- 64372.6
- Isoelectric Point
- 6.41
- Median Abundance (molecules/cell)
- 5213 +/- 1735
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PHO84 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Inorganic phosphate transmembrane transporter involved in phosphate ion transport and polyphosphate metabolism; also has manganese ion transmembrane transporter activity and selenite:proton symporter activity involved in the transport of selenite across the plasma membrane; integral component of the plasma membrane
View computational annotations
Molecular Function
- Manually Curated
- enables manganese ion transmembrane transporter activity (IMP)
- enables phosphate transmembrane transporter activity (IDA, IMP)
- enables selenite:proton symporter activity (IDA, IMP)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in manganese ion transport (IMP)
- involved in phosphate ion transport (IMP, IDA)
- involved in plasma membrane selenite transport (IMP)
- involved in polyphosphate metabolic process (IMP)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- located in fungal-type vacuole (HDA)
- located in plasma membrane (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant fails to grow in low-phosphate medium, does not take up inorganic phosphate, and fails to accumulate polyphosphate; null mutant displays metal resistance; ts mutant has elevated acid phosphatase activity at elevated temperature in the presence of inorganic phosphate; in large-scale studies, null mutant has reduced competitive fitness in a variety of media and has altered resistance to various chemicals
Classical Genetics
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- growth in exponential phase: decreased rate
- growth in exponential phase: increased rate
- lipid particle morphology: abnormal
- metal resistance: increased
- nutrient uptake: absent
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: increased
- small molecule transport: decreased rate
- vegetative growth: absent
- vegetative growth: increased
- conditional
- misexpression
- null
- biofilm formation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- utilization of nitrogen source: decreased rate
- utilization of phosphorus source: decreased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
295 total interactions for 239 unique genes
Physical Interactions
- Affinity Capture-MS: 38
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 1
- PCA: 19
- Protein-RNA: 1
- Proximity Label-MS: 1
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 145
- Phenotypic Enhancement: 10
- Phenotypic Suppression: 22
- Positive Genetic: 22
- Synthetic Growth Defect: 9
- Synthetic Lethality: 6
- Synthetic Rescue: 8
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 25
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.