DBP2 / YNL112W Overview
- Standard Name
- DBP2 1
- Systematic Name
- YNL112W
- SGD ID
- SGD:S000005056
- Feature Type
- ORF , Verified
- Description
- ATP-dependent RNA helicase of the DEAD-box protein family; strong preference for dsRNA; remodels RNP structure for efficient transcription termination; required for assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may suppress transcription from cryptic initiation sites; promotes Mex67p-mediated nuclear export of mRNA by facilitating annealing with antisense RNAs to create dsRNAs; binds G-quadruplex (G4) nucleic acids 2 3 4 5 6 7
- Name Description
- Dead Box Protein 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 546
- Mol. Weight (Da)
- 61007.3
- Isoelectric Point
- 9.03
- Median Abundance (molecules/cell)
- 21619 +/- 7516
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DBP2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATP-dependent RNA helicase involved in messenger ribonucleoprotein (mRNP) complex assembly, rRNA processing, and mRNA decay; binds G-quadruplex (G4) nucleic acids and destabilizes G4RNA structures; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables ATP-dependent activity, acting on RNA (IMP, IDA)
- enables G-quadruplex DNA binding (IDA)
- enables G-quadruplex RNA binding (IDA)
- enables mRNA binding (IDA)
- enables RNA helicase activity (IDA)
- enables snoRNA binding (IDA)
Biological Process
- Manually Curated
- involved in nuclear polyadenylation-dependent mRNA catabolic process (IGI, IMP)
- involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay (IPI)
- involved in rRNA processing (IMP)
- involved in termination of RNA polymerase II transcription (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation confers slow vegetative growth; in a systematic study, null mutant scored as inviable in strain S288C, possibly due to slow growth
Classical Genetics
- null
- reduction of function
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
450 total interactions for 372 unique genes
Physical Interactions
- Affinity Capture-MS: 277
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 4
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- PCA: 17
- Proximity Label-MS: 3
- Reconstituted Complex: 4
- Two-hybrid: 4
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 112
- Phenotypic Enhancement: 1
- Positive Genetic: 10
- Synthetic Growth Defect: 2
- Synthetic Lethality: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.