PIK1 / YNL267W Overview
- Standard Name
- PIK1 1
- Systematic Name
- YNL267W
- SGD ID
- SGD:S000005211
- Aliases
- PIK41 30 , PIK120 30
- Feature Type
- ORF , Verified
- Description
- Phosphatidylinositol 4-kinase; catalyzes the first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; required for autophagosome formation during autophagy and for lipophagy in both stationary phase cells and during nitrogen starvation; may control nonselective autophagy and mitophagy through trafficking of Atg9p; localizes to the nucleus and to the trans-Golgi network 1 2 3 4 5 6 7
- Name Description
- Phosphatidyl Inositol Kinase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1066
- Mol. Weight (Da)
- 119912.6
- Isoelectric Point
- 6.46
- Median Abundance (molecules/cell)
- 2603 +/- 1584
- Half-life (hr)
- 9.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PIK1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 1-phosphatidylinositol 4-kinase involved in phosphatidylinositol phosphorylation; localizes to trans-Golgi network and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables 1-phosphatidylinositol 4-kinase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in cellular response to nitrogen starvation (IMP)
- involved in microlipophagy (IMP)
- involved in phosphatidylinositol phosphate biosynthetic process (IDA, IMP)
- involved in positive regulation of autophagosome assembly (IMP)
Cellular Component
- Manually Curated
- located in nucleus (IDA)
- located in trans-Golgi network (IDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional mutations disturb phosphatidylinositol phosphate pools and cause multiple abnormalities in actin cytoskeleton, Golgi and vacuole morphology, defects in cytokinesis, autophagy, mitophagy, protein trafficking
Classical Genetics
- null
- overexpression
- repressible
- conditional
- actin cytoskeleton morphology: abnormal
- actin cytoskeleton morphology: normal
- autophagy: absent
- auxotrophy
- chemical compound accumulation: decreased
- cytokinesis: absent
- endocytosis: decreased
- endomembrane system morphology: abnormal
- Golgi morphology: abnormal
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- heat sensitivity: normal
- meiosis: decreased
- mitophagy: decreased
- protein secretion: decreased
- protein transport: abnormal
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: normal
- RNA localization: abnormal
- sporulation: absent
- vacuolar morphology: abnormal
- vacuolar transport: abnormal
- vacuolar transport: decreased
- vacuolar transport: normal
- unspecified
- reduction of function
- null
- reduction of function
- repressible
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
369 total interactions for 262 unique genes
Physical Interactions
- Affinity Capture-MS: 23
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 9
- Biochemical Activity: 3
- Co-fractionation: 3
- Co-localization: 3
- Reconstituted Complex: 9
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 3
- Dosage Rescue: 10
- Negative Genetic: 113
- Phenotypic Enhancement: 9
- Phenotypic Suppression: 3
- Positive Genetic: 9
- Synthetic Growth Defect: 96
- Synthetic Lethality: 58
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-06-09
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.