DDL1 / YOR022C Overview
- Standard Name
- DDL1 1
- Systematic Name
- YOR022C
- SGD ID
- SGD:S000005548
- Feature Type
- ORF , Verified
- Description
- DDHD domain-containing phospholipase A1; mitochondrial matrix enzyme with sn-1-specific activity, hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in remodeling of mitochondrial phospholipids; antagonistically regulated by Aft1p and Aft2p; in humans, mutations in DDHD1 and DDHD2 genes cause specific types of hereditary spastic paraplegia, while DDL1-defective yeast share similar phenotypes such as mitochondrial dysfunction and defects in lipid metabolism 1 2 3 4
- Name Description
- DDHD Domain-containing Lipase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 715
- Mol. Weight (Da)
- 81769.0
- Isoelectric Point
- 7.42
- Median Abundance (molecules/cell)
- 657 +/- 344
- Half-life (hr)
- 8.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DDL1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Phospholipase; involved in the metabolism of phospholipids including: phosphatidylcholine, phosphatidylethanolamine, phosphatidic acid and cardiolipin; localizes to the mitochondrion
View computational annotations
Molecular Function
- Manually Curated
- enables phospholipase activity (IDA, IMP, ISS)
Biological Process
- Manually Curated
- involved in cardiolipin metabolic process (IMP)
- involved in phosphatidylethanolamine metabolic process (IMP)
- involved in phospholipid metabolic process (IMP)
Cellular Component
- Manually Curated
- located in mitochondrial matrix (IDA)
- located in mitochondrion (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows decreased competitive fitness on ethanol and on minimal and glycerol media, decreased respiratory respiratory growth on glycerol, lactate, and ethanol, decreased resistance to the toxin yttrium, and increased mutation frequency; heterozygous diploid is haploinsufficient and shows increased global mutation frequency and decreased resistance to the drugs imiprimine and methotrexate; homozygous diploid shows decreased respiratory growth on glycerol, ethanol, and lactate, and decreased resistance to the fermentation inhibitor glycoaldehyde; overexpression leads to increased invasive growth in the homozygous diploid
Classical Genetics
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast DDL1 is homologous to human DDHD1 and DDHD2, and has been used to study hereditary spastic paraplegias
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
75 total interactions for 65 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 4
Genetic Interactions
- Dosage Rescue: 3
- Negative Genetic: 45
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 3
- Positive Genetic: 11
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 5
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.