EXO1 / YOR033C Overview
- Standard Name
- EXO1 1
- Systematic Name
- YOR033C
- SGD ID
- SGD:S000005559
- Aliases
- DHS1 6
- Feature Type
- ORF , Verified
- Description
- 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication 2 3 4 5
- Name Description
- EXOnuclease 1
- Paralog
- DIN7 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- EXO1/YOR033C is located on the right arm of chromosome XV near the centromere between uncharacterized gene YOR032W-A and AKR2 ankyrin repeat-containing protein; coding sequence is 2109 nucleotides long with 24 SNPs, 7 of which cause amino acid polymorphisms; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Exo1p is 702 amino acids long, extremely low in abundance; contains 5 XPG/Rad2 endonuclease domains; phosphorylated on 7 serines; relative distribution to the nucleus increases upon DNA replication stress
- Length (a.a.)
- 702
- Mol. Weight (Da)
- 80172.4
- Isoelectric Point
- 8.49
- Median Abundance (molecules/cell)
- 140 +/- 124
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all EXO1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- 5'-3' exonuclease and 5'-flap endonuclease involved in DNA double-strand break processing, gene conversion at mating-type locus, and mismatch repair; also involved in telomere maintenance and telomeric 3' overhang formation; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables 5'-3' exonuclease activity (IDA)
- enables 5'-flap endonuclease activity (IDA)
Biological Process
- Manually Curated
- involved in DNA double-strand break processing (IGI, IMP)
- involved in gene conversion at mating-type locus (IMP)
- involved in meiotic DNA double-strand break processing (IGI)
- involved in mismatch repair (IMP)
- involved in mitotic G2/M transition checkpoint (IGI)
- involved in telomere maintenance (IGI)
- involved in telomeric 3' overhang formation (IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to MMS and phleomycin; sumoylation of Rad52p and Rfa1p in response to DNA damage is reduced in the null mutant and increased in an activated mutant; in large-scale studies the null mutant displays decreased cell size and altered resistance to various chemicals
Classical Genetics
- null
- cell size: decreased
- chemical compound accumulation: abnormal
- competitive fitness: increased
- heat sensitivity: decreased
- liquid culture appearance: abnormal
- mating response: absent
- mutation frequency: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- starvation resistance: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast EXO1 is homologous to human EXO1, and has been used to study mutations found in patients with primary ovarian insufficiency
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Exo1p interacts physically with proteins involved in DNA repair and meiotic cell cycle; EXO1 interacts genetically with genes involved in DNA repair; the exo1 null mutant is viable, the null mutant of paralog din7 is viable, the exo1 din7 double mutant has not been annotated for phenotype
638 total interactions for 254 unique genes
Physical Interactions
- Affinity Capture-MS: 18
- Affinity Capture-RNA: 1
- Affinity Capture-Western: 14
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Far Western: 2
- Protein-peptide: 1
- Reconstituted Complex: 1
- Two-hybrid: 16
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 32
- Negative Genetic: 155
- Phenotypic Enhancement: 138
- Phenotypic Suppression: 86
- Positive Genetic: 21
- Synthetic Growth Defect: 59
- Synthetic Lethality: 29
- Synthetic Rescue: 61
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- EXO1 transcription is regulated by Sfp1p in response to stress; stability of EXO1 mRNA is upregulated by Npl3p and downregulated by Rrp6p
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-07-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.