PFY1 / YOR122C Overview
- Standard Name
- PFY1 1
- Systematic Name
- YOR122C
- SGD ID
- SGD:S000005648
- Aliases
- CLS5 16 , PRF1
- Feature Type
- ORF , Verified
- Description
- Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS 2 3 4 5 6
- Name Description
- ProFilin of Yeast
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Pfy1p is 126 amino acids long, of average half-life, and high in abundance; contains 10 profilin domains; succinylated on K15, acetylated on K18, phosphorylated on 3 residues, ubiquitinylated on K93; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 126
- Mol. Weight (Da)
- 13674.6
- Isoelectric Point
- 5.59
- Median Abundance (molecules/cell)
- 22053 +/- 6496
- Half-life (hr)
- 10.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all PFY1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Peripheral plasma membrane protein capable of binding actin monomers, PIP2, and proline-rich regions of proteins; involved in sequestering of actin monomers and positive regulation of formin-nucleated actin cable assembly; also localizes to cytosol
View computational annotations
Molecular Function
- Manually Curated
- enables actin monomer binding (IDA, IMP)
- enables phosphatidylinositol-4,5-bisphosphate binding (IMP, IDA)
- enables proline-rich region binding (IDA, IMP)
- enables protein sequestering activity (IDA)
Biological Process
- Manually Curated
- involved in intracellular transport (IGI)
- involved in mitotic actomyosin contractile ring assembly (IMP)
- involved in positive regulation of formin-nucleated actin cable assembly (IMP, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in S288C, non-essential in Sigma1278b and other strain backgrounds; null mutant in a non-essential background has a growth defect, is temperature sensitive, is larger and rounder than wt, with a decreased unipolar budding pattern, altered actin cytoskeleton morphology, delocalized chitin deposition, and is often multinucleate; null mutant has a reduced mating efficiency when cells are mating type alpha; conditional mutant displays increased chromosomal instability; heterozygous diploid null mutant has reduced starvation resistance; overexpression results in a reduced growth rate
Classical Genetics
- null
- actin cytoskeleton morphology: abnormal
- axial budding pattern: abnormal
- axial budding pattern: decreased
- cell shape: abnormal
- cell size: increased
- cellular morphology: abnormal
- chitin deposition: abnormal
- growth in exponential phase: decreased rate
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- inviable
- mating efficiency: decreased
- nuclear import: decreased
- nuclear morphology: abnormal
- protein secretion: delayed
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- resistance to chemicals: decreased
- spore germination: absent
- vegetative growth: decreased
- reduction of function
- conditional
- null
- repressible
- reduction of function
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast PFY1 is homologous to human PFN1, and has been used to study amyotrophic lateral sclerosis type 18
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Pfy1p interacts physically with proteins involved in translation and organelle organization; PFY1 interacts genetically with genes involved in cytoskeleton organization, transcription, and mitosis
1005 total interactions for 748 unique genes
Physical Interactions
- Affinity Capture-Luminescence: 1
- Affinity Capture-MS: 13
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 2
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-purification: 1
- PCA: 3
- Proximity Label-MS: 2
- Reconstituted Complex: 2
- Two-hybrid: 10
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 1
- Dosage Rescue: 18
- Negative Genetic: 673
- Phenotypic Enhancement: 5
- Phenotypic Suppression: 2
- Positive Genetic: 105
- Synthetic Growth Defect: 129
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 17
- Synthetic Rescue: 5
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2004-10-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.