DPB2 / YPR175W Overview
- Standard Name
- DPB2 1
- Systematic Name
- YPR175W
- SGD ID
- SGD:S000006379
- Feature Type
- ORF , Verified
- Description
- Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate 1 2 3 4
- Name Description
- DNA Polymerase B 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 689
- Mol. Weight (Da)
- 78330.9
- Isoelectric Point
- 6.09
- Median Abundance (molecules/cell)
- 2091 +/- 689
- Half-life (hr)
- 11.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DPB2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the DNA polymerase epsilon complex; involved in maintenance of fidelity during DNA replication, error prone translesion synthesis, heterochromatin organization that contributes to chromatin silencing; also localizes to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- contributes to DNA polymerase processivity factor activity (IDA)
- contributes to DNA-directed DNA polymerase activity (IDA)
- contributes to double-stranded DNA binding (IDA)
- contributes to single-stranded DNA binding (IDA)
Biological Process
- Manually Curated
- involved in DNA-templated DNA replication (IDA)
- involved in DNA-templated DNA replication maintenance of fidelity (IMP)
- involved in error-prone translesion synthesis (IMP, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional (ts) mutants grow slowly even at permissive temperature and form abnormally shaped (dumbbell) cells; repression causes defects in cell cycle progression though S phase and elevated chromosome rearrangements and loss
Classical Genetics
- null
- repressible
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
182 total interactions for 85 unique genes
Physical Interactions
- Affinity Capture-MS: 58
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 48
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 7
- Reconstituted Complex: 9
- Two-hybrid: 23
Genetic Interactions
- Dosage Rescue: 4
- Negative Genetic: 6
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 2
- Synthetic Growth Defect: 5
- Synthetic Lethality: 6
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.