PHO4 and PHO2 protein were overexpressed in E. coli and purified respectively. The gel retardation assays showed that PHO4 and PHO2 protein could bind to -401 -289 bp sequence of PHO81 gene promoter respectively. The assays of the mutant PHO2 proteins where the Ser-230 was changed into Asp (GST-2SD) or Ala (GST-2SA) showed the binding patterns were the same as the intact PHO2 protein. Footprinting results located the PHO4 binding site at the 354 -333 bp sequence of PHO81 gene PHO2 binding site at the -341 -334 bp. Therefore the region (-401 -289 bp) contains the upstream activation sequence (UAS) of PHO81 gene and PHO4 protein cooperates with PHO2 in acting on the upstream sequence of PHO81 gene and both of them regulate the expression of PHO81 gene.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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