By use of a selective galactose agar medium containing ethidium bromide, a population analysis of the deinduction kinetics of yeast galactose long-term adaptation mutants (gal 3) has been done. It was first determined that the gal 3 mutation is specific to the yeast galactose system and that induced cultures of gal 3 strains are capable of growth on galactose agar medium containing ethidium bromide, whereas noninduced cultures are not. Population analyses of induced gal 3 strains under going deinduction in the absence of galactose demonstrate that a minimum number of five induction units per cell are required for induction of the galactose system. It is concluded that: these induction units are actively synthesized only in the presence of inducer and are diluted out through cell division; they are stable under nongrowing conditions; they are heterogeneous in nature; at most two of the five minimum units are products of the gal 2 locus; and the other units may be three of one type, one of one type and two of another, or one each of three different types.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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