Reference: Farrelly FW and Finkelstein DB (1984) Complete sequence of the heat shock-inducible HSP90 gene of Saccharomyces cerevisiae. J Biol Chem 259(9):5745-51

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Abstract


We have sequenced the yeast HSP90 gene, which encodes the (apparent) Mr = 90,000 heat shock-inducible protein of this organism. All sequences required for the heat shock-regulated expression of this gene reside on a segment of DNA containing no more than 273 nucleotides 5' to the transcription origin. Transcript mapping reveals that the mature hsp90 mRNA contains a 59-nucleotide 5' untranslated segment, a coding segment of 2130 nucleotides (sufficient to encode a protein of Mr = 81,419), and a 3' untranslated segment of 128 nucleotides. Although the sequences surrounding the coding region of the HSP90 gene are quite similar to those surrounding other sequenced yeast genes, we find as well a limited homology between sequences located 5' to this gene and the putative heat shock-regulatory sequences located 5' to the heat shock-inducible genes of Drosophila.

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Journal Article | Research Support, U.S. Gov't, P.H.S.
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Farrelly FW, Finkelstein DB
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