We have isolated the pgmB gene from Aspergillus nidulans, which encodes a phosphoglucomutase, one of the key enzymes in carbohydrate metabolism. The pgmB gene is located on chromosome VII and its ORF encodes 557 amino acids. Mutant phenotypes were analysed by expression of high levels of pgmB antisense RNA, which lead to a loss of detectable levels of sense RNA. Under conditions of antisense RNA expression, a 30% reduction in the growth rate was observed in comparison to wild-type. On the enzyme level, expression of pgmB antisense RNA resulted in a 35% reduction in total phosphoglucomutase activity. Two pgmB mRNAs were observed under all conditions tested and differ with respect to the location of the poly(A) site. Expression of pgmB driven by the GAL1 promoter in Saccharomyces cerevisiae complemented the growth phenotype of a pgm2delta mutant strain and suppressed the sensitivity of a gcn4delta mutant strain to amino acid starvation in the presence of galactose. Cultivation of A. nidulans in the presence of glucose or galactose as carbon source did not affect transcription of pgmB. However, amino acid starvation conditions resulted in a six-fold reduction in the level of pgmB mRNA, while genes for amino acid biosynthesis showed increased transcription. Transcription of pgmB was low during hyphal growth and in the sexual phase of development, but was significantly increased during the asexual stage of the A. nidulans life cycle.

• PMID: 10660061
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Comparative Study | Journal Article | Research Support, Non-U.S. Gov't

Authors

Hoffmann B, LaPaglia SK, Kübler E, Andermann M, Eckert SE, Braus GH

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