Labile altered forms of the his4 protein from Saccharomyces cerevisiae were studied in extracts of mutant strains or in extracts subjected to proteolysis. Fragments of the his4 protein were detected by immunoautoradiography in high salt extracts of his4 deletion strains having histidinol dehydrogenase activity but lacking two of the other enzyme activities associated with the trifunctional his4 protein. A form of the protein altered by proteolysis was also detected in low salt crude extracts by immunodiffusion and shown to possess histidinol dehydrogenase function by activity staining of the precipitin lines. These immunological data indicated that histidinol dehydrogenase can function independently of the other two activities in extracts of deletion strains or in extracts where the wild type his4 protein has undergone proteolytic cleavage. The data also indicated that high concentration in (NH4)2SO4 had a substantial stabilizing effect on the his4 protein and on fragments of the his4 protein from mutant strains.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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