Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog. The Mek1 protein associates with meiotic chromosomes and colocalizes with the Red1 protein, which is a component of meiotic chromosome cores. Mek1 and Red1 interact physically in meiotic cells, as demonstrated by coimmunoprecipitation and the two-hybrid protein system. Hop1, another protein associated with meiotic chromosome cores, also interacts with Mek1 but only in the presence of Red1. Red1 displays Mek1-dependent phosphorylation, both in vitro and in vivo, and Mek1 kinase activity is necessary for Mek1 function in vivo. Fluorescent in situ hybridization analysis indicates that Mek1-mediated phosphorylation of Red1 is required for meiotic sister-chromatid cohesion, raising the possibility that cohesion is regulated by protein phosphorylation.
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
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MEK1 | enables | protein serine/threonine kinase activity | happens during meiotic prophase I, has input RED1 | IDA | SGD | 2019-11-06 |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Interactor | Interactor | Assay | Annotation | Action | Modification | |
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HOP1 | MEK1 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
HOP1 | MEK1 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue | |
RED1 | MEK1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
RED1 | MEK1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
RED1 | MEK1 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
RED1 | MEK1 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue |