Reference: Bailis JM and Roeder GS (1998) Synaptonemal complex morphogenesis and sister-chromatid cohesion require Mek1-dependent phosphorylation of a meiotic chromosomal protein. Genes Dev 12(22):3551-63

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Abstract


Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog. The Mek1 protein associates with meiotic chromosomes and colocalizes with the Red1 protein, which is a component of meiotic chromosome cores. Mek1 and Red1 interact physically in meiotic cells, as demonstrated by coimmunoprecipitation and the two-hybrid protein system. Hop1, another protein associated with meiotic chromosome cores, also interacts with Mek1 but only in the presence of Red1. Red1 displays Mek1-dependent phosphorylation, both in vitro and in vivo, and Mek1 kinase activity is necessary for Mek1 function in vivo. Fluorescent in situ hybridization analysis indicates that Mek1-mediated phosphorylation of Red1 is required for meiotic sister-chromatid cohesion, raising the possibility that cohesion is regulated by protein phosphorylation.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Bailis JM, Roeder GS
Primary Lit For
HOP1 | RED1 | MEK1 | Synaptonemal complex

Gene Ontology Annotations 1 entry for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
MEK1enablesprotein serine/threonine kinase activityhappens during meiotic prophase I, has input RED1IDASGD2019-11-06
Showing 1 to 1 of 1 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 6 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
HOP1MEK1Two-hybridmanually curatedHit-BaitNo Modification
HOP1MEK1Biochemical Activitymanually curatedHit-Baitphosphorylated residue
RED1MEK1Affinity Capture-Westernmanually curatedHit-BaitNo Modification
RED1MEK1Affinity Capture-Westernmanually curatedBait-HitNo Modification
RED1MEK1Two-hybridmanually curatedHit-BaitNo Modification
RED1MEK1Biochemical Activitymanually curatedHit-Baitphosphorylated residue
Showing 1 to 6 of 6 entries