We report genetic and biochemical evidence that the RNA polymerase II carboxy-terminal domain (CTD) interacts with a large multisubunit complex that contains TATA-binding protein (TBP) and is an integral part of the transcription initiation complex. The isolation and characterization of extragenic suppressors of S. cerevisiae RNA polymerase II CTD truncation mutations led us to identify SRB2, SRB4, SRB5, and SRB6 as genes involved in CTD function in vivo. SRB2 was previously isolated and shown to encode a 23 kd TBP-binding protein. The four SRB proteins and a portion of cellular TBP are components of a high molecular weight multisubunit complex that is tightly bound to RNA polymerase II. This SRB-TBP complex binds specifically to recombinant CTD protein. In vitro transcription and template commitment assays confirm that SRB2 and SRB5 are components of a functional preinitiation complex and are required for efficient transcription initiation.
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| RPO21 | SRB5 | Reconstituted Complex | manually curated | Bait-Hit | No Modification | |
| SPT15 | RPO21 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| SRB2 | RPO21 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| SRB4 | RPO21 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| SRB4 | SPT15 | Co-purification | manually curated | Bait-Hit | No Modification | |
| SRB4 | SRB2 | Co-purification | manually curated | Bait-Hit | No Modification | |
| SRB4 | SRB5 | Co-purification | manually curated | Bait-Hit | No Modification | |
| SRB6 | RPO21 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| SRB6 | SRB4 | Co-purification | manually curated | Hit-Bait | No Modification |