Studies were undertaken to elucidate the role of the GAL4 locus in regulating the galactose catabolic enzymes in Saccharomyces cerevisiae. The GAL4 locus has been proposed to have a regulatory function since GAL4 mutants are pleiotropic, lacking epimerase, galactokinase, transferase and galactose permease activities. This pleiotropic effect could be explained if the GAL4 gene codes for a peptide required for above enzyme activities. To study if the GAL4 gene codes for a structural component of the above enzymes, a temperature sensitive mutation mapping close to or within the GAL4 gene (gal4-1ts) was isolated. The results suggest that the GAL4 locus does not code for a polypeptide common to the transferase, epimerase, galactokinase, and permease enzymes since these enzymes extracted from the gal4-1ts mutant did not differ qualitatively in thermolability and temperature optima from those of the wild-type. It was found that in the gal4-1ts mutant the synthesis of the epimerase is temperature sensitive and that the GAL4 gene product does not have any effect on the in vivo stability of epimerase.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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