In eukaryotic cells the TATA-binding protein (TBP) associates with other proteins known as TBP-associated factors (TAFs) to form multisubunit transcription factors important for gene expression by all three nuclear RNA polymerases. Computer searching of the complete Saccharomyces cerevisiae genome revealed five previously unidentified yeast genes with significant sequence similarity to known human and Drosophila RNA polymerase II TAFs. Each of these genes is essential for viability. A sixth essential gene (FUN81) has previously been noted to be similar to human TAFII18. Coimmunoprecipitation experiments show that all six proteins are associated with TBP, demonstrating that they are true TAFs. Furthermore, these proteins are present in complexes containing the TAFII130 subunit, indicating that they are components of TFIID. Based on their predicted molecular weights, these genes have been designated TAF67, TAF61(68), TAF40, TAF23(25), TAF19(FUN81), and TAF17. Yeast TAF61 is significantly larger than its higher eukaryotic homologues, and deletion analysis demonstrates that the evolutionarily conserved, histone-like domain is sufficient and necessary to support viability.
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| TAF10 | inviable | classical genetics | null Allele: taf10-Δ | Other | ||
| TAF11 | inviable | classical genetics | null Allele: taf11-Δ | Other | ||
| TAF13 | inviable | classical genetics | null Allele: taf13-Δ | Other | ||
| TAF7 | inviable | classical genetics | null Allele: taf7-Δ | Other | ||
| TAF9 | inviable | classical genetics | null Allele: taf9-Δ | Other | ||
| TAF12 | inviable | classical genetics | null Allele: taf12-Δ | Other |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| SPT15 | TAF9 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | TAF13 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | TAF10 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | TAF11 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | TAF12 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | TAF7 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| TAF1 | TAF9 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| TAF12 | TAF1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |