Leza MA and Elion EA (1999)

Abstract

In the absence of a successful mating, pheromone-arrested Saccharomyces cerevisiae cells reenter the mitotic cycle through a recovery process that involves downregulation of the mating mitogen-activated protein kinase (MAPK) cascade. We have isolated a novel gene, POG1, whose promotion of recovery parallels that of the MAPK phosphatase Msg5. POG1 confers alpha-factor resistance when overexpressed and enhances alpha-factor sensitivity when deleted in the background of an msg5 mutant. Overexpression of POG1 inhibits alpha-factor-induced G1 arrest and transcriptional repression of the CLN1 and CLN2 genes. The block in transcriptional repression occurs at SCB/MCB promoter elements by a mechanism that requires Bck1 but not Cln3. Genetic tests strongly argue that POG1 promotes recovery through upregulation of the CLN2 gene and that the resulting Cln2 protein promotes recovery primarily through an effect on Ste20, an activator of the mating MAPK cascade. A pog1 cln3 double mutant displays synthetic mutant phenotypes shared by cell-wall integrity and actin cytoskeleton mutants, with no synthetic defect in the expression of CLN1 or CLN2. These and other results suggest that POG1 may regulate additional genes during vegetative growth and recovery.

Download Citation (.nbib)

Reference Type: Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors: Leza MA, Elion EA
Primary Lit For: STE20 | POG1 | CLN1 | CLN2 | CLN2-CDC28 kinase complex
Additional Lit For: CLN3 | BCK1 | MSG5
Review For

Gene Ontology Annotations 9 entries for 2 genes

Gene/Complex	Qualifier	Gene Ontology Term	Annotation Extension	Evidence	Source	Assigned On
POG1	involved in	re-entry into mitotic cell cycle after pheromone arrest		IMP	SGD	2024-03-22
POG1	involved in	positive regulation of transcription by RNA polymerase II	part of re-entry into mitotic cell cycle after pheromone arrest	IMP	SGD	2020-05-20
POG1	involved in	positive regulation of transcription by RNA polymerase II	has input CLN1	IMP	SGD	2020-05-20
POG1	involved in	positive regulation of transcription by RNA polymerase II	has input CLN2	IMP	SGD	2020-05-20
POG1	involved in	re-entry into mitotic cell cycle after pheromone arrest		IGI with STE20	SGD	2017-02-27
POG1	involved in	re-entry into mitotic cell cycle after pheromone arrest		IGI with CLN2	SGD	2017-02-27
POG1	involved in	re-entry into mitotic cell cycle after pheromone arrest		IGI with MSG5	SGD	2017-02-27
CLN2	involved in	re-entry into mitotic cell cycle after pheromone arrest		IGI with POG1	SGD	2017-02-27
CLN2	involved in	re-entry into mitotic cell cycle after pheromone arrest		IGI with STE20	SGD	2017-02-27

Gene/Complex

Qualifier

Gene Ontology Term

Annotation Extension

Evidence

Source

Assigned On

POG1

involved in

re-entry into mitotic cell cycle after pheromone arrest

IMP

SGD

2024-03-22

POG1

involved in

positive regulation of transcription by RNA polymerase II

part of re-entry into mitotic cell cycle after pheromone arrest

IMP

SGD

2020-05-20

POG1

involved in

positive regulation of transcription by RNA polymerase II

has input CLN1

IMP

SGD

2020-05-20

POG1

involved in

positive regulation of transcription by RNA polymerase II

has input CLN2

IMP

SGD

2020-05-20

POG1

involved in

re-entry into mitotic cell cycle after pheromone arrest

IGI with STE20

SGD

2017-02-27

POG1

involved in

re-entry into mitotic cell cycle after pheromone arrest

IGI with CLN2

SGD

2017-02-27

POG1

involved in

re-entry into mitotic cell cycle after pheromone arrest

IGI with MSG5

SGD

2017-02-27

CLN2

involved in

re-entry into mitotic cell cycle after pheromone arrest

IGI with POG1

SGD

2017-02-27

CLN2

involved in

re-entry into mitotic cell cycle after pheromone arrest

IGI with STE20

SGD

2017-02-27

Phenotype Annotations 4 entries for 1 gene

Gene	Phenotype	Experiment Type	Mutant Information	Strain Background	Details
POG1	pheromone sensitivity: decreased	classical genetics	overexpression	W303	Assay: halo assay Details: decreased zone of alpha-factor induced growth inhibition in an sst1 null background
POG1	pheromone-induced cell cycle arrest: decreased	classical genetics	overexpression	W303	Details: decreased alpha-factor induced G1 phase arrest observed in an sst1 null background
POG1	protein/peptide accumulation: increased Reporter: Cln2p	classical genetics	overexpression	W303	Treatment: alpha-factor Details: blocks alpha-factor induced repression of Cln2p in an sst1 null mutant
POG1	shmoo formation: decreased	classical genetics	overexpression	W303	Details: decreased alpha-factor induced mating projection formation observed in an sst1 null background