Reference: Fiebig KM, et al. (1999) Folding intermediates of SNARE complex assembly. Nat Struct Biol 6(2):117-23

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Abstract


SNARE (soluble NSF attachment protein receptor) proteins assemble into a stable complex essential for vesicle-membrane fusion. To further understand SNARE function we have used solution nuclear magnetic resonance (NMR) spectroscopy to characterize three assembly states of a yeast SNARE complex: first, the 'closed' conformation of Sso1; second, the binary complex of Sso1 and Sec9; and third, the ternary complex of Sso1, Sec9 and Snc1. Sec9 and Snc1 are unstructured in isolation. Sso1 likely consists of a four helix bundle formed by part of the C-terminal Hcore domain and the N-terminal H(A)H(B)H(C) domain, and this bundle is flanked on both sides by large flexible regions. Sso1 switches to an 'open' state when its Hcore domain binds Sec9. Conformational switching of the Hcore domain, via H(A)H(B)H(C), may provide a key regulatory mechanism in SNARE assembly. Formation of binary and ternary complexes induces additional alpha-helical structure in previously unstructured regions. Our data suggest a directed assembly process beginning distal to the membrane surfaces and proceeding toward them, bringing membranes into close proximity and possibly leading to membrane fusion.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Fiebig KM, Rice LM, Pollock E, Brunger AT
Primary Lit For
SNC1 | SEC9 | SSO1 | Vesicular SNARE complex SSO1-SEC9-SNC1
Additional Lit For
Vesicular SNARE complex SSO2-SEC9-SNC1

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 3 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
SEC9SSO1Co-crystal Structuremanually curatedHit-BaitNo Modification
SNC1SEC9Co-crystal Structuremanually curatedHit-BaitNo Modification
SNC1SSO1Co-crystal Structuremanually curatedHit-BaitNo Modification
Showing 1 to 3 of 3 entries