Reference: Fiebig KM, et al. (1999)
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Abstract
SNARE (soluble NSF attachment protein receptor) proteins assemble into a stable complex essential for vesicle-membrane fusion. To further understand SNARE function we have used solution nuclear magnetic resonance (NMR) spectroscopy to characterize three assembly states of a yeast SNARE complex: first, the 'closed' conformation of Sso1; second, the binary complex of Sso1 and Sec9; and third, the ternary complex of Sso1, Sec9 and Snc1. Sec9 and Snc1 are unstructured in isolation. Sso1 likely consists of a four helix bundle formed by part of the C-terminal Hcore domain and the N-terminal H(A)H(B)H(C) domain, and this bundle is flanked on both sides by large flexible regions. Sso1 switches to an 'open' state when its Hcore domain binds Sec9. Conformational switching of the Hcore domain, via H(A)H(B)H(C), may provide a key regulatory mechanism in SNARE assembly. Formation of binary and ternary complexes induces additional alpha-helical structure in previously unstructured regions. Our data suggest a directed assembly process beginning distal to the membrane surfaces and proceeding toward them, bringing membranes into close proximity and possibly leading to membrane fusion.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Fiebig KM,
Rice LM,
Pollock E,
Brunger AT
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- SNC1 | SEC9 | SSO1 | Vesicular SNARE complex SSO1-SEC9-SNC1
- Vesicular SNARE complex SSO2-SEC9-SNC1
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
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Annotation Extension |
Evidence |
Method |
Source |
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
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Gene |
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Disease Ontology Term |
Disease Ontology Term ID |
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
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Post-translational Modifications
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Analyze ID |
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Interactor Systematic Name |
Interactor |
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SGA score |
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Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| SEC9 | SSO1 | Co-crystal Structure | manually curated | Hit-Bait | No Modification |
| SNC1 | SEC9 | Co-crystal Structure | manually curated | Hit-Bait | No Modification |
| SNC1 | SSO1 | Co-crystal Structure | manually curated | Hit-Bait | No Modification |
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |