Signals from transcriptional activators to the general mRNA transcription apparatus are communicated by factors associated with RNA polymerase II or the TATA-binding protein (TBP). Currently, little is known about how gene-specific transcription repressors communicate with RNA polymerase II. We have analyzed the requirements for repression by the saccharomyces cerevisiae Leu3 protein (Leu3p) in a reconstituted transcription system. We have identified a complex form of TBP which is required for communication of the repressing signal. This TFIID-like complex contains a known TBP-associated protein, Mot1p, which has been implicated in the repression of a subset of yeast genes by genetic analysis. Leu3p-dependent repression can be reconstituted with purified Mot1p and recombinant TBP. In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor.
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| CCL1 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| RPO21 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| RPO21 | TFG1 | Co-purification | manually curated | Hit-Bait | No Modification | |
| SPT15 | SUA7 | Co-purification | manually curated | Bait-Hit | No Modification | |
| SPT15 | MOT1 | Co-purification | manually curated | Bait-Hit | No Modification | |
| SPT15 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| SRB2 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| SRB4 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| SRB5 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification | |
| SRB6 | TFB4 | Co-purification | manually curated | Hit-Bait | No Modification |