The Saccharomyces cerevisiae succinate-ubiquinone reductase or succinate dehydrogenase (SDH) is a tetramer of non-equivalent subunits encoded by the SDH1, SDH2, SDH3, and SDH4 genes. In most organisms, SDH contains one or two endogenous b-type hemes. However, it is widely believed that the yeast SDH does not contain heme. In this report, we demonstrate the presence of a stoichiometric amount of cytochrome b562 in the yeast SDH. The cytochrome is detected as a peak present in fumarate-oxidized, dithionite-reduced mitochondria. The peak is centered at 562 nm and is present at a heme:covalent FAD molar ratio of 0.92+/-0.11. The cytochrome is not detectable in mitochondria isolated from SDH3 and SDH4 deletion strains. These observations strongly support our conclusion that cytochrome b562 is a component of the yeast SDH.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| Mitochondrial respiratory chain complex II | involved in | tricarboxylic acid cycle | IDA | ComplexPortal | 2018-01-11 | |
| Mitochondrial respiratory chain complex II | located in | mitochondrial inner membrane | IDA | ComplexPortal | 2018-01-11 | |
| Mitochondrial respiratory chain complex II | enables | succinate dehydrogenase (quinone) activity | IDA | ComplexPortal | 2018-01-11 | |
| Mitochondrial respiratory chain complex II | involved in | mitochondrial electron transport, succinate to ubiquinone | IDA | ComplexPortal | 2018-01-11 | |
| SDH4 | involved in | mitochondrial electron transport, succinate to ubiquinone | TAS | SGD | 2013-08-07 |