Reference: Acker J, et al. (1994) A 14.4 KDa acidic subunit of human RNA polymerase II with a putative leucine-zipper. DNA Seq 4(5):329-31

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Abstract


The cDNA of a small subunit (hRPB14.4) of RNA polymerase II (or B) from HeLa cells has been cloned. A 127 residue peptide sequence (calculated molecular weight of 14,478; isoelectric point of 3.7) was deduced and compared to that of the homologous subunit of Saccharomyces cerevisiae polymerase (ABC23, encoded by the RPB6/RPO26 gene). About 50% of the total residues were found to be conserved between yeast and man, with the C-terminal two-third being the most conserved (72% identity). A putative leucine-zipper comprising four properly spaced leucine residues, but not preceded by a basic domain, was identified near the C-terminal end of both proteins.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Acker J, Wintzerith M, Vigneron M, Kedinger C
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