Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG-CoA reductase kinase-A (HRK-A) from Brassica oleracea on Western blots. HRK-A was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. HRK-A was not recognized by antibodies against plant, mammalian and Saccharomyces cerevisiae relatives of RKIN1, i.e. wheat PKABA1, rat AMP-activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG-CoA reductase kinase-A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.

• PMID: 8543048
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Ball KL, Barker J, Halford NG, Hardie DG

Primary Lit For

Additional Lit For

SNF1

Review For