Reference: Piper MD, et al. (2000) Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem 275(40):30987-95

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Abstract


One-carbon metabolism in yeast is an essential process that relies on at least one of three one-carbon donor molecules: serine, glycine, or formate. By a combination of genetics and biochemistry we have shown how cells regulate the balance of one-carbon flow between the donors by regulating cytoplasmic serine hydroxymethyltransferase activity in a side reaction occurring in the presence of excess glycine. This control governs the level of 5,10-methylene tetrahydrofolate (5,10-CH(2)-H(4)folate) in the cytoplasm, which has a direct role in signaling transcriptional control of the expression of key genes, particularly those encoding the unique components of the glycine decarboxylase complex (GCV1, GCV2, and GCV3). Based on these and other observations, we propose a model for how cells balance the need to supplement their one-carbon pools when charged folates are limiting or when glycine is in excess. We also propose that under normal conditions, cytoplasmic 5,10-CH(2)-H(4)folate is mainly directed to generating methyl groups via methionine, whereas one-carbon units generated from glycine in mitochondria are more directed to purine biosynthesis. When glycine is in excess, 5, 10-CH(2)-H(4)folate is decreased, and the regulation loop shifts the balance of generation of one-carbon units into the mitochondrion.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Piper MD, Hong SP, Ball GE, Dawes IW
Primary Lit For
GCV1 | GCV3 | GCV2 | MET13 | Glycine decarboxylase multienzyme complex

Gene Ontology Annotations 4 entries for 3 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
GCV3involved inone-carbon metabolic processIGI with SHM2SGD2013-08-07
GCV1involved inone-carbon metabolic processIGI with SHM2SGD2013-08-07
GCV1involved inone-carbon metabolic processIMPSGD2013-08-07
GCV2involved inone-carbon metabolic processIGI with SHM2SGD2013-08-07
Showing 1 to 4 of 4 entries