Reference: Sengupta SM, et al. (2001) The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling. J Biol Chem 276(16):12636-44

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Abstract


Interactions of the yeast chromatin-remodeling complexes SWI/SNF and RSC with nucleosomes were probed using site-specific DNA photoaffinity labeling. 5 S rDNA was engineered with photoreactive nucleotides incorporated at different sites in DNA to scan for the subunits of SWI/SNF in close proximity to DNA when SWI/SNF is bound to the 5 S nucleosome or to the free 5 S rDNA. The Swi2/Snf2 and Snf6 subunits of SWI/SNF were efficiently cross-linked at several positions in the nucleosome, whereas only Snf6 was efficiently cross-linked when SWI/SNF was bound to free DNA. DNA photoaffinity labeling of RSC showed that the Rsc4 subunit is in close proximity to nucleosomal DNA and not when RSC is bound to free DNA. After remodeling, the Swi2/Snf2 and Rsc4 subunits are no longer detected near the nucleosomal DNA and are evidently displaced from the surface of the nucleosome, indicating significant changes in SWI/SNF and RSC contacts with DNA after remodeling.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Sengupta SM, VanKanegan M, Persinger J, Logie C, Cairns BR, Peterson CL, Bartholomew B
Primary Lit For
SNF6 | RSC4 | SNF2 | SWI1 | SWI/SNF chromatin remodelling complex

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 4 entries for 5 genes

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InteractorInteractorAssayAnnotationActionModification
RSC1RSC2Co-purificationmanually curatedHit-BaitNo Modification
RSC3RSC2Co-purificationmanually curatedHit-BaitNo Modification
RSC4RSC2Co-purificationmanually curatedHit-BaitNo Modification
STH1RSC2Co-purificationmanually curatedHit-BaitNo Modification
Showing 1 to 4 of 4 entries