Chromatin insulators are defined as transcriptionally neutral elements that prevent negative or positive influence from extending across chromatin to a promoter. Here we show that yeast subtelomeric anti-silencing regions behave as boundaries to telomere-driven silencing and also allow discontinuous propagation of silent chromatin. These two facets of insulator activity, boundary and silencing discontinuity, can be recapitulated by tethering various transcription activation domains to tandem sites on DNA. Importantly, we show that these insulator activities do not involve direct transcriptional activation of the reporter promoter. These findings predict that certain promoters behave as insulators and partition genomes in functionally independent domains.

• PMID: 11258704
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Fourel G, Boscheron C, Revardel E, Lebrun E, Hu YF, Simmen KC, Müller K, Li R, Mermod N, Gilson E

Primary Lit For

REB1 | TBF1

Additional Lit For

Review For