The structure of an actively transcribing complex, containing yeast RNA polymerase II with associated template DNA and product RNA, was determined by electron crystallography. Nucleic acid, in all likelihood the "transcription bubble" at the active center of the enzyme, occupies a previously noted 25 A channel in the protein structure. Details are indicative of a roughly 90 degrees bend of the DNA between upstream and downstream regions. The DNA apparently lies entirely on one face of the polymerase, rather than passing through a hole to the opposite side, as previously suggested.

• PMID: 10499796
• DOI full text
• PubMed
• PubTator

Reference Type

Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S. | Research Support, U.S. Gov't, P.H.S.

Authors

Poglitsch CL, Meredith GD, Gnatt AL, Jensen GJ, Chang WH, Fu J, Kornberg RD

Primary Lit For

RPB9 | RPC10 | RPB5 | RPO21 | RPB3 | RPO26 | RPB11 | RPB10 | RPB8 | RPB2 | DNA-directed RNA polymerase II complex ... Show all Show fewer

Additional Lit For

Review For