Here we report that RNA polymerase (pol) III transcription is repressed in response to DNA damage by downregulation of TFIIIB, the core component of the pol III transcriptional machinery. Protein kinase CK2 transduces this stress signal to TFIIIB. CK2 associates with and normally activates the TATA binding protein (TBP) subunit of TFIIIB. The beta regulatory subunit of CK2 binds to TBP and is required for high TBP-associated CK2 activity and pol III transcription in unstressed cells. Transcriptional repression induced by DNA damage requires CK2 and coincides with downregulation of TBP-associated CK2 and dissociation of catalytic subunits from TBP-CK2 complexes. Therefore, CK2 is the terminal effector in a signaling pathway that represses pol III transcription when genome integrity is compromised.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| CKB1 | involved in | regulation of transcription by RNA polymerase III | IDA | SGD | 2013-08-07 | |
| CKB1 | involved in | regulation of transcription by RNA polymerase I | IDA | SGD | 2013-08-07 | |
| CKB1 | involved in | DNA damage response | IDA | SGD | 2013-08-07 | |
| CKA1 | involved in | regulation of transcription by RNA polymerase III | IDA | SGD | 2013-08-07 | |
| CKA1 | involved in | regulation of transcription by RNA polymerase I | IDA | SGD | 2013-08-07 | |
| CKA1 | involved in | DNA damage response | IDA | SGD | 2013-08-07 | |
| CKB2 | involved in | regulation of transcription by RNA polymerase I | IDA | SGD | 2013-08-07 | |
| CKB2 | involved in | regulation of transcription by RNA polymerase III | IDA | SGD | 2013-08-07 | |
| CKB2 | involved in | DNA damage response | IDA | SGD | 2013-08-07 | |
| CKA2 | involved in | DNA damage response | IDA | SGD | 2013-08-07 |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| CKB1 | BRF1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| SPT15 | CKA2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SPT15 | CKB1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SPT15 | CKB2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| SPT15 | CKB1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification | |
| SPT15 | CKA2 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue |