Reference: Kemp HA and Sprague GF (2003) Far3 and five interacting proteins prevent premature recovery from pheromone arrest in the budding yeast Saccharomyces cerevisiae. Mol Cell Biol 23(5):1750-63

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Abstract


In budding yeast, diffusible mating pheromones initiate a signaling pathway that culminates in several responses, including cell cycle arrest. Only a handful of genes required for the interface between pheromone response and the cell cycle have been identified, among them FAR1 and FAR3; of these, only FAR1 has been extensively characterized. In an effort to learn about the mechanism by which Far3 acts, we used the two-hybrid method to identify interacting proteins. We identified five previously uncharacterized open reading frames, dubbed FAR7, FAR8, FAR9, FAR10, and FAR11, that cause a far3-like pheromone arrest defect when disrupted. Using two-hybrid and coimmunoprecipitation analysis, we found that all six Far proteins interact with each other. Moreover, velocity sedimentation experiments suggest that Far3 and Far7 to Far11 form a complex. The phenotype of a sextuple far3far7-far11 mutant is no more severe than any single mutant. Thus, FAR3 and FAR7 to FAR11 all participate in the same pathway leading to G1 arrest. These mutants initially arrest in response to pheromone but resume budding after 10 h. Under these conditions, wild-type cells fail to resume budding even after several days whereas far1 mutant cells resume budding within 1 h. We conclude that the FAR3-dependent arrest pathway is functionally distinct from that which employs FAR1.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Kemp HA, Sprague GF
Primary Lit For
FAR1 | VPS64 | FAR7 | FAR11 | FAR8 | FAR10 | FAR3 | FAR complex

Gene Ontology Annotations


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Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

Phenotype Annotations 9 entries for 7 genes


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
VPS64pheromone sensitivity: abnormal
classical geneticsnull
Allele: vps64-Δ
OtherDetails: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest
FAR10pheromone sensitivity: abnormal
classical geneticsnull
Allele: far10-Δ
OtherDetails: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest
FAR8pheromone sensitivity: abnormal
classical geneticsnull
Allele: far8-Δ
OtherDetails: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest
FAR11pheromone sensitivity: abnormal
classical geneticsnull
Allele: far11-Δ
OtherDetails: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest
FAR3pheromone sensitivity: abnormal
classical geneticsnull
Allele: far3-Δ
OtherDetails: mutants arrest transiently but recover more rapidly in the presence of alpha factor, yet have intact signal transduction (FUS1 induction)
FAR7pheromone sensitivity: abnormal
classical geneticsnull
Allele: far7-Δ
OtherDetails: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest
FAR1pheromone sensitivity: abnormal
classical geneticsnull
Allele: far1-Δ
OtherDetails: mutants unable to arrest in the presence of alpha factor yet have intact signal transduction (FUS1 induction)
FAR1pheromone-induced cell cycle arrest: absent
classical geneticsnull
Allele: far1-Δ
OtherDetails: display little or no arrest, reentering the cell cycle immediately upon exposure to pheromone
FAR3recovery from pheromone-induced cell cycle arrest: increased rate
classical geneticsnull
Allele: far3-Δ
OtherTreatment: alpha-factor, 0.1 ug/ml
Details: budding resumes 10 hours after initial arrest
Showing 1 to 9 of 9 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 7 entries for 6 genes

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InteractorInteractorAssayAnnotationActionModification
FAR10FAR3Two-hybridmanually curatedHit-BaitNo Modification
FAR3FAR11Two-hybridmanually curatedBait-HitNo Modification
FAR7FAR3Two-hybridmanually curatedHit-BaitNo Modification
FAR7FAR8Two-hybridmanually curatedBait-HitNo Modification
FAR7VPS64Two-hybridmanually curatedBait-HitNo Modification
FAR8FAR3Two-hybridmanually curatedHit-BaitNo Modification
VPS64FAR3Two-hybridmanually curatedHit-BaitNo Modification
Showing 1 to 7 of 7 entries