In budding yeast, diffusible mating pheromones initiate a signaling pathway that culminates in several responses, including cell cycle arrest. Only a handful of genes required for the interface between pheromone response and the cell cycle have been identified, among them FAR1 and FAR3; of these, only FAR1 has been extensively characterized. In an effort to learn about the mechanism by which Far3 acts, we used the two-hybrid method to identify interacting proteins. We identified five previously uncharacterized open reading frames, dubbed FAR7, FAR8, FAR9, FAR10, and FAR11, that cause a far3-like pheromone arrest defect when disrupted. Using two-hybrid and coimmunoprecipitation analysis, we found that all six Far proteins interact with each other. Moreover, velocity sedimentation experiments suggest that Far3 and Far7 to Far11 form a complex. The phenotype of a sextuple far3far7-far11 mutant is no more severe than any single mutant. Thus, FAR3 and FAR7 to FAR11 all participate in the same pathway leading to G1 arrest. These mutants initially arrest in response to pheromone but resume budding after 10 h. Under these conditions, wild-type cells fail to resume budding even after several days whereas far1 mutant cells resume budding within 1 h. We conclude that the FAR3-dependent arrest pathway is functionally distinct from that which employs FAR1.
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
---|
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
---|---|---|---|---|---|---|
VPS64 | pheromone sensitivity: abnormal | classical genetics | null Allele: vps64-Δ | Other | Details: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest | |
FAR10 | pheromone sensitivity: abnormal | classical genetics | null Allele: far10-Δ | Other | Details: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest | |
FAR8 | pheromone sensitivity: abnormal | classical genetics | null Allele: far8-Δ | Other | Details: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest | |
FAR11 | pheromone sensitivity: abnormal | classical genetics | null Allele: far11-Δ | Other | Details: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest | |
FAR3 | pheromone sensitivity: abnormal | classical genetics | null Allele: far3-Δ | Other | Details: mutants arrest transiently but recover more rapidly in the presence of alpha factor, yet have intact signal transduction (FUS1 induction) | |
FAR7 | pheromone sensitivity: abnormal | classical genetics | null Allele: far7-Δ | Other | Details: mutants display abnormal pheromone induced arrest yet have intact signal transduction (FUS1 induction); results with a sextuple (far3 far7 to far11) mutant indicate premature recovery from G1 arrest | |
FAR1 | pheromone sensitivity: abnormal | classical genetics | null Allele: far1-Δ | Other | Details: mutants unable to arrest in the presence of alpha factor yet have intact signal transduction (FUS1 induction) | |
FAR1 | pheromone-induced cell cycle arrest: absent | classical genetics | null Allele: far1-Δ | Other | Details: display little or no arrest, reentering the cell cycle immediately upon exposure to pheromone | |
FAR3 | recovery from pheromone-induced cell cycle arrest: increased rate | classical genetics | null Allele: far3-Δ | Other | Treatment: alpha-factor, 0.1 ug/ml Details: budding resumes 10 hours after initial arrest |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
---|
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Interactor | Interactor | Assay | Annotation | Action | Modification | |
---|---|---|---|---|---|---|
FAR10 | FAR3 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
FAR3 | FAR11 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
FAR7 | FAR3 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
FAR7 | FAR8 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
FAR7 | VPS64 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
FAR8 | FAR3 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
VPS64 | FAR3 | Two-hybrid | manually curated | Hit-Bait | No Modification |