Formation and nuclear export of 60 S pre-ribosomes requires many factors including the heterodimeric Noc1-Noc2 and Noc2-Noc3 complexes. Here, we report another Noc complex with a specific role in 40 S subunit biogenesis. This complex consists of Noc4p, which exhibits the conserved Noc domain and is homologous to Noc1p, and Nop14p, a nucleolar protein with a role in 40 S subunit formation. Moreover, noc4 thermosensitive mutants are defective in 40 S biogenesis, and rRNA processing is inhibited at early cleavage sites A(0), A(1), and A(2). Using a fluorescence-based visual assay for 40 S subunit export, we observe a strong nucleolar accumulation of the Rps2p-green fluorescent protein reporter in noc4 ts mutants, but 60 S subunit export was normal. Thus, Noc4p and Nop14p form a novel Noc complex with a specific role in nucleolar 40 S subunit formation and subsequent export to the cytoplasm.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| NOP14 | part of | Noc4p-Nop14p complex | IPI with NOC4 | SGD | 2013-08-07 | |
| NOC4 | part of | Noc4p-Nop14p complex | IPI with NOP14 | SGD | 2013-08-07 | |
| NOC4 | involved in | rRNA processing | IMP | SGD | 2013-08-07 | |
| NOC4 | involved in | ribosomal small subunit biogenesis | IMP | SGD | 2013-08-07 | |
| NOC4 | located in | nucleolus | IDA | SGD | 2013-08-07 |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| MAK21 | NOC2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| MAK21 | NOC2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| MAK21 | NOC3 | Co-fractionation | manually curated | Bait-Hit | No Modification | |
| MAK21 | NOC4 | Co-fractionation | manually curated | Bait-Hit | No Modification | |
| NOP14 | NOC4 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| NOP14 | NOC4 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |