Autophagy, pexophagy, and the Cvt pathway are processes that deliver hydrolytic enzymes and substrates to the yeast vacuole/lysosome via double-membrane cytosolic vesicles. Whereas these pathways operate under different nutritional conditions, they all employ common machinery with only a few specific factors assisting in the choice of the delivery program and the membrane source for the sequestering vesicle. We found that the YKR020w gene product is essential for Cvt vesicle formation but not for pexophagy or induction of autophagy. Autophagosomes in the ykr020wdelta mutant, however, have a reduced size. We demonstrate that Ykr020 is a subunit of the Vps fifty-three tethering complex, composed of Vps52, Vps53, and Vps54, which is required for retrograde traffic from the early endosome back to the late Golgi, and for this reason we named it Vps51. This complex participates in a fusion event together with Tlg1 and Tlg2, two SNAREs also shown to be necessary for Cvt vesicle assembly. In particular, those factors are essential to correctly target the prApe1-Cvt19-Cvt9 complex to the preautophagosomal structure, the site of Cvt vesicle formation.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| VPS52 | involved in | positive regulation of macroautophagy | IMP | SGD | 2015-10-12 | |
| VPS51 | involved in | vesicle organization | IMP | SGD | 2013-08-07 | |
| VPS51 | involved in | positive regulation of macroautophagy | IMP | SGD | 2015-10-12 | |
| VPS51 | part of | GARP complex | IPI with VPS54, VPS52, VPS53 | SGD | 2019-09-09 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| VPS52 | autophagy: abnormal | classical genetics | null Allele: vps52-Δ | Other | Media: nitrogen starvation Temperature: elevated temperature, 37 °C Details: autophagosomes smaller than wt accumulate although total number is unchanged; in a conditional vam3 mutant background to stabilize cytosolic autophagosomes | |
| VPS51 | autophagy: abnormal | classical genetics | null Allele: vps51-Δ | Other | Media: nitrogen starvation Temperature: elevated temperature, 37 °C Details: autophagosomes smaller than wt accumulate although total number is unchanged; in a conditional vam3 mutant background to stabilize cytosolic autophagosomes | |
| VPS52 | autophagy: decreased | classical genetics | null Allele: vps52-Δ | Other | Media: nitrogen starvation Details: autophagy assayed by delivery of cytoplasmic Pho8p to vacuole | |
| VPS51 | autophagy: decreased | classical genetics | null Allele: vps51-Δ | Other | Media: nitrogen starvation Details: autophagy assayed by delivery of cytoplasmic Pho8p to vacuole | |
| VPS52 | protein/peptide modification: absent Reporter: Ape1p (API) | classical genetics | null Allele: vps52-Δ | S288C | Media: nutrient-rich medium Details: pro-aminopeptidase I (API) is not processed to mature API | |
| VPS54 | protein/peptide modification: absent Reporter: Ape1p (API) | classical genetics | null Allele: vps54-Δ | S288C | Media: nutrient-rich medium Details: pro-aminopeptidase I (API) is not processed to mature API | |
| VPS51 | protein/peptide modification: absent Reporter: Ape1p (API) | classical genetics | null Allele: vps51-Δ | S288C | Media: nutrient-rich medium Details: pro-aminopeptidase I (API) is not processed to mature API | |
| VPS53 | protein/peptide modification: absent Reporter: Ape1p (API) | classical genetics | null Allele: vps53-Δ | S288C | Media: nutrient-rich medium Details: pro-aminopeptidase I (API) is not processed to mature API | |
| VPS54 | protein/peptide modification: normal Reporter: Ape1p (API) | classical genetics | null Allele: vps54-Δ | S288C | Media: nitrogen starvation Details: pro-aminopeptidase I (API) is normally processed to mature API after delivery to the vacuole via autophagy | |
| VPS52 | protein/peptide modification: normal Reporter: Ape1p (API) | classical genetics | null Allele: vps52-Δ | S288C | Media: nitrogen starvation Details: pro-aminopeptidase I (API) is normally processed to mature API after delivery to the vacuole via autophagy |
Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| VPS51 | TLG1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| VPS51 | VPS51 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| VPS51 | VPS52 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification | |
| VPS51 | VPS52 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| VPS51 | VPS53 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification | |
| VPS51 | VPS54 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |