The Saccharomyces cerevisiae cyclin-dependent kinase (CDK) Bur1 (Sgv1) may be homologous to mammalian Cdk9, which functions in transcriptional elongation. Although Bur1 can phosphorylate the Rpb1 carboxy-terminal domain (CTD) kinase in vitro, it has no strong specificity within the consensus heptapeptide YSPTSPS for Ser2 or Ser5. BUR1 mutants are sensitive to the drugs 6-azauracil and mycophenolic acid and interact genetically with the elongation factors Ctk1 and Spt5. Chromatin immunoprecipitation experiments show that Bur1 and its cyclin partner Bur2 are recruited to transcription elongation complexes, cross-linking to actively transcribing genes. Interestingly, Bur1 shows reduced cross-linking to transcribed regions downstream of polyadenylation sites. In addition, bur1 mutant strains have a reduced cross-linking ratio of RNA polymerase II at the 3' end of genes relative to promoter regions. Phosphorylation of CTD serines 2 and 5 appears normal in mutant cells, suggesting that Bur1 is not a significant source of cotranscriptional Rpb1 phosphorylation. These results show that Bur1 functions in transcription elongation but may phosphorylate a substrate other than the CTD.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SGV1 | involved in | positive regulation of transcription elongation by RNA polymerase II | IMP | SGD | 2015-11-14 | |
| SGV1 | involved in | positive regulation of transcription elongation by RNA polymerase II | IGI with SPT5 | SGD | 2015-11-14 | |
| SGV1 | involved in | positive regulation of transcription elongation by RNA polymerase II | IGI with CTK1 | SGD | 2015-11-14 |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Interactor | Interactor | Assay | Annotation | Action | Modification | |
|---|---|---|---|---|---|---|
| CTK1 | RPO21 | Biochemical Activity | manually curated | Bait-Hit | phosphorylated residue | |
| KIN28 | RPO21 | Biochemical Activity | manually curated | Bait-Hit | phosphorylated residue | |
| RPO21 | SGV1 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue | |
| RPO21 | SSN3 | Biochemical Activity | manually curated | Hit-Bait | phosphorylated residue |