In order to study interactions among proteins involved in Candida albicans morphorgenesis, C.albicans genomic DNA was digested with Sau3AI and fused to activating domain(AD) of LEXA to construct an AD-fused C.albicans genomic DNA expression library. The library contained 7.7x10(4) independent clones and included 1.2x10(5) kb DNA which was 10 fold of the C.albicans whole genomic DNA. The CRK1 gene encodes a CDC2-related protein, that was involved in morphorgenesis of C.albicans. The Crk1 fused to DNA binding domain of LexA was used as the bait to screen the LexA library. Among 9x10(5) transformants, eight Leu and LacZ double positive clones were selected. With restriction enzyme analysis, three kinds of fragments were identified. The clones NJ1, NJ2, NJ3 were sequenced and analyzed with BLAST program. It was found that NJ1, NJ2, NJ3 were homologs of S.cerevisiae gene STI1, RET2 and NFI1, respectively. The kinase domain of Crk1(Crk1N)interacted weakly with protein encoded by NJ1, while the noncatalytic domain of Crk1(Crk1C) interacted strongly with proteins encoded by NJ2 and NJ3. The interaction of NJ3 and Crk1C was verified by the coimmunoprecipition of NJ3-HA with Crk1C-LexA. These proteins may be involved in maturation, transport, localization, and activity regulation of the Crk1.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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