Reference: Smardon AM and Kane PM (2007)
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Abstract
The RAVE complex is required for stable assembly of the yeast vacuolar proton-translocating ATPase (V-ATPase) during both biosynthesis of the enzyme and regulated reassembly of disassembled V(1) and V(0) sectors. It is not yet known how RAVE effects V-ATPase assembly. Previous work has shown that V(1) peripheral or stator stalk subunits E and G are critical for binding of RAVE to cytosolic V(1) complexes, suggesting that RAVE may play a role in docking of the V(1) peripheral stalk to the V(0) complex at the membrane. Here we provide evidence for an interaction between the RAVE complex and V(1) subunit C, another subunit that has been assigned to the peripheral stalk. The C subunit is unique in that it is released from both V(1) and V(0) sectors during disassembly, suggesting that subunit C may control the regulated assembly of the V-ATPase. Mutants lacking subunit C have assembly phenotypes resembling that of RAVE mutants. Both are able to assemble V(1)/V(0) complexes in vivo, but these complexes are highly unstable in vitro, and V-ATPase activity is extremely low. We show that in the absence of the RAVE complex, subunit C is not able to stably assemble with the vacuolar ATPase. Our data support a model where RAVE, through its interaction with subunit C, is facilitating V(1) peripheral stalk subunit interactions with V(0) during V-ATPase assembly.
- Reference Type
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Journal Article |
Research Support, N.I.H., Extramural |
Research Support, Non-U.S. Gov't
- Authors
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Smardon AM,
Kane PM
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- RAV1 | RAVE complex
- RAV2 | SKP1 | VMA5 | VMA4 | VMA10
Gene Ontology Annotations
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| RAV1 | VMA4 | Two-hybrid | manually curated | Bait-Hit | No Modification |
| RAV2 | RAV1 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| RAV2 | RAV1 | Two-hybrid | manually curated | Bait-Hit | No Modification |
| SKP1 | RAV1 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| VMA10 | RAV1 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| VMA2 | RAV1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| VMA5 | RAV1 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| VMA5 | RAV2 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| VMA5 | RAV1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
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