Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.

• PMID: 19182523
• DOI full text
• PubMed
• PubTator

Reference Type

Journal Article

Authors

Krick R, Mühe Y, Prick T, Bredschneider M, Bremer S, Wenzel D, Eskelinen EL, Thumm M

Primary Lit For

Additional Lit For

SWH1

Review For

VAC8 | NVJ1