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Reference: Thaker YR, et al. (2009) Association of the eukaryotic V1VO ATPase subunits a with d and d with A. FEBS Lett 583(7):1090-5

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Abstract

Owing to the complex nature of V(1)V(O) ATPases, identification of neighboring subunits is essential for mechanistic understanding of this enzyme. Here, we describe the links between the V(1) headpiece and the V(O)-domain of the yeast V(1)V(O) ATPase via subunit A and d as well as the V(O) subunits a and d using surface plasmon resonance and fluorescence correlation spectroscopy. Binding constants of about 60 and 200 nM have been determined for the a-d and d-A assembly, respectively. The data are discussed in light of subunit a and d forming a peripheral stalk, connecting the catalytic A(3)B(3) hexamer with V(O).

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Thaker YR, Hunke C, Yau YH, Shochat SG, Li Y, Grüber G
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