An integration plasmid pA15-PET for expression and secretion of beta-Endoglucanase I (EG I ) in yeast was constructed by insertion of EG I cDNA between yeast alcohol dehydrogenase promoter and terminator region. The plasmid contained part of yeast rDNA sequence, which was used as a homologous fragment for integration. The EG I cDNA was introduced into an engineered brewing yeast BE9711 containing alpha-acetolactate decarboxylase (alpha-ALDC) encoding gene and integrated onto its rDNA sequence of chromosomal DNA by co-transformation of pA15-PET and a YEP type plasmid pA15TXR carrying G418 resistance. The stable engineered brewing yeast expressing intracellulase alpha-ALDC and extracellular EG I simultaneously were obtained.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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