Passage of E. coli RNA polymerase through an intrinsic transcription terminator, which encodes an RNA hairpin followed by a stretch of uridine residues, results in quick dissociation of the elongation complex. We show that folding of the hairpin disrupts the three upstream base pairs of the 8 bp RNA:DNA hybrid, a major stability determinant in the complex. Shortening the weak rU:dA hybrid from 8 nt to 5 nt causes dissociation of the complex. During termination, the hairpin does not directly compete for base pairing with the 8 bp hybrid. Thus, melting of the hybrid seems to result from spatial restrictions in RNA polymerase that couple the hairpin formation with the disruption of the hybrid immediately downstream from the stem. Our results suggest that a similar mechanism disrupts elongation complexes of yeast RNA polymerase II in vitro.

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Reference Type

Journal Article | Research Support, U.S. Gov't, P.H.S.

Authors

Komissarova N, Becker J, Solter S, Kireeva M, Kashlev M

Primary Lit For

DNA-directed RNA polymerase II complex

Additional Lit For

RPB9 | RPB7 | RPB4 | RPC10 | RPB5 | RPO21 | RPB3 | RPO26 | RPB11 | RPB10 | RPB8 | ... Show allRPB2 Show fewer

Review For