The conidiophore stalk-less1 (COS1) gene encodes a novel transcription factor in Magnaporthe oryzae, and mutation of COS1 (M2942) resulted in developmental failure of conidiophores. COS1 putatively encodes a 491-amino-acid protein, which contains four multiple adjacent C2H2-type zinc-finger domains. The motifs are homologous to the zinc-finger protein Azf1 of Saccharomyces cerevisiae. Here, we report that the differences of expression profile between M2942 and the wild-type isolate Y34 by RNA-Sequence. DNA sequence analysis of promoter regions of those of COS1-dependent genes showed enrichment in the DNA sequence AAAAGAAA (A4GA3), the putative COS1-binding motif. Gel shift experiments showed that COS1 binds to DNA elements with A4GA3 motif. These suggest that many of the COS1-dependent transcripts may be regulated directly by COS1 binding.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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