Reference: van der Felden J, et al. (2014) The transcription factors Tec1 and Ste12 interact with coregulators Msa1 and Msa2 to activate adhesion and multicellular development. Mol Cell Biol 34(12):2283-93

Reference Help

Abstract


In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TEA consensus sequences [TCSs]) that can be further combined with Ste12 binding sites (pheromone response elements [PREs]) for cooperative DNA binding. The activity of Tec1-Ste12 complexes is known to be under negative control of the Dig1 and Dig2 (Dig1/2) transcriptional corepressors that confer regulation by upstream signaling pathways. Here, we found that Tec1 and Ste12 can associate with the transcriptional coregulators Msa1 and Msa2 (Msa1/2), which were previously found to associate with the cell cycle transcription factor complexes SBF (Swi4/Swi6 cell cycle box binding factor) and MBF (Mbp1/Swi6 cell cycle box binding factor) to control G1-specific transcription. We further show that Tec1-Ste12-Msa1/2 complexes (i) do not contain Swi4 or Mbp1, (ii) assemble at single TCSs or combined TCS-PREs in vitro, and (iii) coregulate genes involved in adhesive and filamentous growth by direct promoter binding in vivo. Finally, we found that, in contrast to Dig proteins, Msa1/2 seem to act as coactivators that enhance the transcriptional activity of Tec1-Ste12. Taken together, our findings add an additional layer of complexity to our understanding of the control mechanisms exerted by the evolutionarily conserved TEA domain and Ste12-like transcription factors.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
van der Felden J, Weisser S, Brückner S, Lenz P, Mösch HU
Primary Lit For
TEC1 | STE12 | MSA1 | MSA2 | SBF transcription complex | MBP transcription complex
Additional Lit For
FLO11 | MSB2 | MBP1 | SWI4

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 16 entries for 6 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
MBP1MSA1Affinity Capture-Westernmanually curatedHit-BaitNo Modification
MSA2MBP1Affinity Capture-Westernmanually curatedBait-HitNo Modification
STE12MSA1Reconstituted Complexmanually curatedBait-HitNo Modification
STE12MSA2Reconstituted Complexmanually curatedBait-HitNo Modification
STE12MSA1Affinity Capture-Westernmanually curatedHit-BaitNo Modification
STE12MSA2Affinity Capture-Westernmanually curatedHit-BaitNo Modification
SWI4MSA1Affinity Capture-Westernmanually curatedBait-HitNo Modification
SWI4MSA2Affinity Capture-Westernmanually curatedBait-HitNo Modification
TEC1MSA2Two-hybridmanually curatedHit-BaitNo Modification
TEC1MSA1Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 10 of 16 entries