Reference: van der Felden J, et al. (2014)
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Abstract
In Saccharomyces cerevisiae and related yeast species, the TEA transcription factor Tec1, together with a second transcription factor, Ste12, controls development, including cell adhesion and filament formation. Tec1-Ste12 complexes control target genes through Tec1 binding sites (TEA consensus sequences [TCSs]) that can be further combined with Ste12 binding sites (pheromone response elements [PREs]) for cooperative DNA binding. The activity of Tec1-Ste12 complexes is known to be under negative control of the Dig1 and Dig2 (Dig1/2) transcriptional corepressors that confer regulation by upstream signaling pathways. Here, we found that Tec1 and Ste12 can associate with the transcriptional coregulators Msa1 and Msa2 (Msa1/2), which were previously found to associate with the cell cycle transcription factor complexes SBF (Swi4/Swi6 cell cycle box binding factor) and MBF (Mbp1/Swi6 cell cycle box binding factor) to control G1-specific transcription. We further show that Tec1-Ste12-Msa1/2 complexes (i) do not contain Swi4 or Mbp1, (ii) assemble at single TCSs or combined TCS-PREs in vitro, and (iii) coregulate genes involved in adhesive and filamentous growth by direct promoter binding in vivo. Finally, we found that, in contrast to Dig proteins, Msa1/2 seem to act as coactivators that enhance the transcriptional activity of Tec1-Ste12. Taken together, our findings add an additional layer of complexity to our understanding of the control mechanisms exerted by the evolutionarily conserved TEA domain and Ste12-like transcription factors.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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van der Felden J,
Weisser S,
Brückner S,
Lenz P,
Mösch HU
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- TEC1 | STE12 | MSA1 | MSA2 | SBF transcription complex | MBP transcription complex
- FLO11 | MSB2 | MBP1 | SWI4
Gene Ontology Annotations
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Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
Assigned On |
Reference |
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Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
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Disease Ontology Term |
Disease Ontology Term ID |
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Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
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Evidence |
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Post-translational Modifications
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Genetic Interactions
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Evidence ID |
Analyze ID |
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Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
Allele |
Assay |
Annotation |
Action |
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SGA score |
P-value |
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Note |
Physical Interactions
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| MBP1 | MSA1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| MSA2 | MBP1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| STE12 | MSA1 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| STE12 | MSA2 | Reconstituted Complex | manually curated | Bait-Hit | No Modification |
| STE12 | MSA1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| STE12 | MSA2 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| SWI4 | MSA1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| SWI4 | MSA2 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| TEC1 | MSA2 | Two-hybrid | manually curated | Hit-Bait | No Modification |
| TEC1 | MSA1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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Complement ID |
Locus ID |
Gene |
Species |
Gene ID |
Strain background |
Direction |
Details |
Source |
Reference |