Cadmium (Cd) is one of the most toxic heavy metals. It is of great environmental concern and its toxicity has been investigated in a variety of cells. In this study, we elucidated the toxic effects of Cd in cells of yeast (Saccharomyces cerevisiae). Our results showed that Cd²⁺ (0.05-5.0 mmol·L^-1) significantly inhibited yeast cell growth, and the inhibitory effect was positively correlated with Cd²⁺ concentrations. Cd²⁺ caused loss of yeast cell viability in a concentration- and duration-dependent manner. Intracellular reactive oxygen species (ROS) and Ca²⁺ levels increased in yeast cells after exposure to 5.0 mmol·L^-1 Cd for 6 h. Cd²⁺-caused cell viability loss was blocked by antioxidants (0.5 mmol·L^-1 ascorbic acid or 500 U·mL^-1 catalase) or Ca²⁺ antagonists (0.5 mmol·L^-1 ethylene glycol tetraacetic acid or 0.5 mmol·L^-1 LaCl₃). Moreover, a collapse of mitochondrial membrane potential (ΔΨ_m) was observed in Cd²⁺-treated yeast cells. These results indicate that Cd-induced yeast cell killing was associated with the elevation of intracellular ROS and Ca²⁺ levels and also the loss of ΔΨ_m.

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Journal Article

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Wang X, Yi M, Liu H, Han Y, Yi H

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