The eukaryotic 43S preinitiation complex (PIC), bearing initiator methionyl transfer RNA (Met-tRNA_i) in a ternary complex (TC) with eukaryotic initiation factor 2 (eIF2)-GTP, scans the mRNA leader for an AUG codon in favorable context. AUG recognition evokes rearrangement from an open PIC conformation with TC in a "P_OUT" state to a closed conformation with TC more tightly bound in a "P_IN" state. eIF1 binds to the 40S subunit and exerts a dual role of enhancing TC binding to the open PIC conformation while antagonizing the P_IN state, necessitating eIF1 dissociation for start codon selection. Structures of reconstituted PICs reveal juxtaposition of eIF1 Loop 2 with the Met-tRNA_i D loop in the P_IN state and predict a distortion of Loop 2 from its conformation in the open complex to avoid a clash with Met-tRNA_i We show that Ala substitutions in Loop 2 increase initiation at both near-cognate UUG codons and AUG codons in poor context. Consistently, the D71A-M74A double substitution stabilizes TC binding to 48S PICs reconstituted with mRNA harboring a UUG start codon, without affecting eIF1 affinity for 40S subunits. Relatively stronger effects were conferred by arginine substitutions; and no Loop 2 substitutions perturbed the rate of TC loading on scanning 40S subunits in vivo. Thus, Loop 2-D loop interactions specifically impede Met-tRNA_i accommodation in the P_IN state without influencing the P_OUT mode of TC binding; and Arg substitutions convert the Loop 2-tRNA_i clash to an electrostatic attraction that stabilizes P_IN and enhances selection of poor start codons in vivo.

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Journal Article | Research Support, N.I.H., Intramural

Authors

Thakur A, Hinnebusch AG

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