Reference: Zhao J, et al. (2018) Facile recycling of Escherichia coli and Saccharomyces cerevisiae cells from suspensions using magnetic modification method and mechanism analysis. Colloids Surf B Biointerfaces 169:1-9

Reference Help

Abstract


Cell recycling for catalysis using whole cell significantly reduced the cost of the catalyst, therefore a simple and rapid method for cell recovery from the reaction system was very important. A facile method for cell modification and recycling was developed by simply mixing the carboxyl-functionalized Fe3O4 magnetic nanoparticles (MNPs) and cell suspension. The mode microbes Escherichia coli and Saccharomyces cerevisiae were easily modified by directly adsorbing MNPs on their surface, which facilitated rapid separation and recycling of cells from suspensions with the aid of magnetic field. It was found that the pH value and ionic strength of cell suspensions played a major role on the modification. Reducing pH value or raising ionic strength facilitated the aggregation of MNPs onto cell surface due to the compression of electrical double layer of MNPs and cells. Interestingly, E. coli might exhibit distinct mechanism of MNP-magnetic modification from S. cerevisiae according to FESEM images, and explained as the different properties of surface zeta potential of the two microbes. The mechanism of modification and the interaction between MNPs and the cell wall structure of E. coli and S. cerevisiae were analyzed. The binding was mainly contributed as the high specific surface area and high surface energy of MNPs, and the decrease of electrostatic repulsive force between MNPs and cells caused by the compression of the interfacial electrical double layer around MNPs and cells. In addition, the hydrogen bonding interaction between the hydroxyl groups of cell wall polysaccharides and the carboxyl groups of MNPs might be supplementary in the binding of MNPs and cells. This work provided a detailed understanding of binding mode in the level of cell structure.

Reference Type
Journal Article
Authors
Zhao J, Lin M, Chen G
Primary Lit For
Additional Lit For
Review For

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Disease Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, or SPELL.

Regulator Target Direction Regulation Of Happens During Method Evidence

Post-translational Modifications


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Site Modification Modifier Reference

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Assay Annotation Action Modification Source Reference

Functional Complementation Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Species Gene ID Strain background Direction Details Source Reference